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. 2019 Nov 4:7:e7941.
doi: 10.7717/peerj.7941. eCollection 2019.

MHC class I allele diversity in cynomolgus macaques of Vietnamese origin

Shuting Huang #  1 , Xia Huang #  1 , Shuang Li  1 , Mingjun Zhu  1 , Min Zhuo  1
Affiliations

MHC class I allele diversity in cynomolgus macaques of Vietnamese origin

Shuting Huang et al. PeerJ. .

Abstract

Cynomolgus macaques (Macaca fascicularis, Mafa) have been used as important experimental animal models for carrying out biomedical researches. The results of biomedical experiments strongly depend on the immunogenetic background of animals, especially on the diversity of major histocompatibility complex (MHC) alleles. However, there is much less information available on the polymorphism of MHC class I genes in cynomolgus macaques, than is currently available for humans. In this study, we have identified 40 Mafa-A and 60 Mafa-B exons 2 and 3 sequences from 30 unrelated cynomolgus macaques of Vietnamese origin. Among these alleles, 28 are novel. As for the remaining 72 known alleles, 15 alleles are shared with other cynomolgus macaque populations and 32 are identical to alleles previously reported in other macaque species. A potential recombination event was observed between Mafa-A1*091:02 and Mafa-A1*057:01. In addition, the Mafa-A1 genes were found to be more diverse than human HLA-A and the functional residues for peptide binding sites (PBS) or TCR binding sites (TBS) in Mafa-A1 have greater variability than that for non-PBS or non-TBS regions. Overall, this study provides important information on the diversity of Mafa-A and Mafa-B alleles from Vietnamese origin, which may help researchers to choose the most appropriate animals for their studies.

Keywords: Cynomolgus macaque; Diversity; Major histocompatibility complex.

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Conflict of interest statement

The authors declare there are no competing interests.

Figures

Figure 1
Figure 1. Summary of MHC class I-A and -B alleles identified from 30 cynomolgusmacaques of Vietnamese origin.
Figure 2
Figure 2. The nucleotide sequences alignment of Mafa-A1*036:04, Mafa-A1*057:01 and Mafa-A1*091:02.
Figure 3
Figure 3. Phylogenetic analysisof exon 2 (A) and exon 3 (B) of A1*036,A1*57 and A1*091 alleles from cynomolgus and rhesusmacaques.
Figure 4
Figure 4. The diversity of amino acid residues at 54 functional positions relativeto PBS or TBS in 67 Mafa-A1 of Vietnamese origin.
See this image and copyright information in PMC

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