Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Nov;62(11):4167-72.
doi: 10.1128/JVI.62.11.4167-4172.1988.

Transition from initiation to elongation in protein-primed phi 29 DNA replication: salt-dependent stimulation by the viral protein p6

L Blanco  1 , A Bernad, M Salas
Affiliations

Transition from initiation to elongation in protein-primed phi 29 DNA replication: salt-dependent stimulation by the viral protein p6

L Blanco et al. J Virol. 1988 Nov.

Abstract

The transition step from the p3-dAMP initiation complex to the first elongated products, p3-(dAMP)2 and p3-(dAMP)3, requires a dATP concentration higher than that needed for the initiation reaction or for the further elongation of the p3-(dAMP)3 complex. The elongation in phi 29 DNA-protein p3 replication in vitro was strongly inhibited by salt. Under inhibitory salt concentration, the viral protein p6 greatly stimulated phi 29 DNA-protein p3 replication. The effect of protein p6 was not on the rate of elongation but on the amount of elongated product, stimulating the transition from initiation to formation of the first elongation products.

PubMed Disclaimer

References

    1. Eur J Biochem. 1972 Dec 4;31(2):367-71 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Jan;85(2):314-8 - PubMed
    1. J Mol Biol. 1978 Feb 25;119(2):269-91 - PubMed
    1. Methods Enzymol. 1980;65(1):499-560 - PubMed
    1. Virology. 1980 Jul 15;104(1):84-96 - PubMed

Publication types

MeSH terms