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. 2019 Sep;26(5):151-157.
doi: 10.21315/mjms2019.26.5.15. Epub 2019 Nov 4.

Human Papillomavirus Infection, p16INK4a Expression and Genetic Alterations in Vietnamese Cervical Neuroendocrine Cancer

To Van Ta  1 , Quang Ngoc Nguyen  1 , Van-Long Truong  2 , Toan Trung Tran  1 , Hung Phi Nguyen  1 , Linh Dieu Vuong  1
Affiliations

Human Papillomavirus Infection, p16INK4a Expression and Genetic Alterations in Vietnamese Cervical Neuroendocrine Cancer

To Van Ta et al. Malays J Med Sci. 2019 Sep.

Abstract

Neuroendocrine cervical cancer is a rare subtype of cervical cancer with a highly aggressive malignancy. This study was conducted to analyse the human papillomavirus (HPV) infection and molecular abnormalities in Vietnamese neuroendocrine carcinomas of the uterine cervix. HPV genotyping and p53 mutations were examined using polymerase chain reaction (PCR)-based direct sequencing. Mutations of epidermal growth factor receptor (EGFR), Kirsten rat sarcoma (KRAS), neuroblastoma RAS viral oncogene homolog (NRAS) and v-Raf murine sarcoma viral oncogene homolog B (BRAF) were identified using commercial kits. Four high-risk HPV genotypes were identified in 26 (86.7%) out of a total of 30 tumours. The prevalence of HPV 16, 18, 31 and 45 was 20.0%, 50.0%, 20.0% and 36.7%, respectively. Overexpression of p16INK4a was observed in 93.3% of cases and was significantly correlated with high-risk HPV infections. Furthermore, p53 and NRAS mutations were detected in five (16.7%) and one (3.3%) cases, respectively, whereas no EGFR, KRAS or BRAF mutations were observed. These results demonstrate that high-risk HPV infection may be an important oncogenic factor for the development and progression of cervical neuroendocrine carcinoma.

Keywords: HPV; NRAS mutation; neuroendocrine cervical cancer; p16INK4a; p53 mutation.

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Conflict of interest statement

Conflict of Interest None.

Figures

Figure 1
Figure 1
Representative photographs of H&E staining (upper panel) and p16INK4a IHC staining analysis (lower panel). Photographs were taken at 200× magnification SCNC = small cell neuroendocrine carcinoma; LCNC = large cell neuroendocrine carcinoma
See this image and copyright information in PMC

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