Short-term biological variation of serum thyroid hormones concentrations in clinically healthy cats

Abstract

Thyroid disease is common in cats, but little is known about the biologic variability of serum thyroid hormone concentrations and its impact on diagnostic utility in either healthy cats or cats with thyroid disease. The purpose of this study was to determine the biological variation, index of individuality, and reference change values for thyroid hormones and thyroid-stimulating hormone (TSH) in clinically healthy cats. Serum samples for analysis of total thyroxine (T₄), triiodothyronine (T₃), free T₄ by dialysis, and TSH were obtained weekly for 6 wk from 10 healthy cats, then frozen until single-batch analyzed. Data were evaluated for outliers, and we determined the CV within individual cats (CV_I) and between individual cats (CV_G) for each hormone and the variation between duplicates or analytical variation (CV_A). The index of individuality and reference change values for each hormone were then calculated. Serum concentrations of total T₄, free T₄, T₃, and TSH all showed greater variation between cats (CV_G) than within cats (CV_I). Total and free T₄ had an intermediate index of individuality (1.1 and 1.2, respectively), suggesting that these hormones would be best evaluated by a combination of their population-based reference intervals and reference change values. Serum TSH concentrations had high index of individuality (1.8), suggesting this hormone would be best evaluated with reference change values rather than the population-based reference interval. Total T₃ also had a high calculated index of individuality (1.8); however, T₃ had high ratio of analytical variation (CV_A) to within cat variation (CV_I), so RCV could not be accurately calculated. This study demonstrates that clinically normal cats show considerable interindividual biological variation in serum thyroid hormone and TSH concentrations, whereas the intraindividual variability in hormone concentrations is much narrower. This suggests that for all serum thyroid hormones, but especially serum TSH and T₃ concentrations, comparing individual cat's hormone results to a population-based reference interval may be misleading, especially in those with early or subclinical thyroid disease. Clinicians might improve the diagnosis of feline thyroid disease by establishing baseline concentrations of T₄, free T₄, T₃, and TSH for individual cats (ideally when healthy) and applying reference change values to subsequent measurements.

Keywords: Index of individuality; Population-based reference intervals; Reference change value; Subject-based reference intervals; Thyroid-stimulating hormone.