Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2019 Oct 29:10:1222.
doi: 10.3389/fphar.2019.01222. eCollection 2019.

Isobologram Analysis: A Comprehensive Review of Methodology and Current Research

Ruo-Yue Huang  1 , Linlin Pei  1 , QuanJin Liu  1 , Shiqi Chen  1 , Haibo Dou  1 , Gang Shu  1 , Zhi-Xiang Yuan  1 , Juchun Lin  1 , Guangneng Peng  1 , Wei Zhang  1 , Hualin Fu  1
Affiliations
Review

Isobologram Analysis: A Comprehensive Review of Methodology and Current Research

Ruo-Yue Huang et al. Front Pharmacol. .

Abstract

Drug combination is a common method for clinical disease treatment. Whether the combination of drugs is reasonable often affects the result of the disease treatment. Many methods have been used to evaluate interaction between drugs to date. Isobologram analysis has been mathematically proven and widely used to evaluate drug interactions. In this paper, the principle of isobologram analysis and its application in drug interaction evaluation are summarized. The applications of the similar cotoxicity coefficient and fractional inhibitory concentration index in the evaluation of drug interaction are also reviewed. This work is expected to evaluate the effect of formulations scientifically and provide scientific judgment standards for the development of formulations and clinical drug compatibility.

Keywords: application; drug interaction; isobologram analysis; principle; synergy.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Classification of drug interactions. (A) is the classification of drug interactions. (B) is the isobologram of the interaction between two drugs. (C) is the isobologram of the combination of three drugs in Figure 1.
Figure 2
Figure 2
Examples of rectangular hyperbolic dose-effect curves. (A) is the example of rectangular hyperbolic dose-effect curves. (B) is the isobologram of the interaction between two drugs.
Figure 3
Figure 3
The typical subadditive and superadditive isoboles.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Ahmadi-Mahmoodabadi N., Nasehi M., Emam Ghoreishi M., Zarrindast M.-R. (2016). Synergistic effect between prelimbic 5-HT3 and CB1 receptors on memory consolidation deficit in adult male Sprague–Dawley rats: an isobologram analysis. Neuroscience, 317, 173–183. 10.1016/j.neuroscience.2015.12.010 - DOI - PubMed
    1. Ajiboye T. O. (2018). 2-(2-Nitrovinyl) furan exacerbates oxidative stress response of Escherichia coli to bacteriostatic and bactericidal antibiotics. Microb. Pathog. 116, 130–134. 10.1016/j.micpath.2018.01.010 - DOI - PubMed
    1. An-Kuo C., Chong-Chi C., Yu-Wen C., Jhi-Joung W., Ching-Hsia H. (2018). Phentolamine Reverses Epinephrine-Enhanced Skin Antinociception of Dibucaine in Rats. Anesth. Analg. 128, 1336–1343. 10.1213/ANE.0000000000003421 - DOI - PubMed
    1. Bansal S. K., Singh K. V., Sharma H. (2015). Synergistic efficacy of Solanum xanthocarpum and Withania somnifera on larvae of mosquito vector species. J. Environ. Biol. 36, 633–638.
    1. Basting R. T., Spindola H. M., Sousa I. M. d. O., Queiroz N. d. C. A., et al. , (2019). Pterodon pubescens and Cordia verbenacea association promotes a synergistic response in antinociceptive model and improves the anti-inflammatory results in animal models. Biomed. Pharmacother. 112, 108693. 10.1016/j.biopha.2019.108693 - DOI - PubMed

LinkOut - more resources