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Comparative Study
. 2020 Jan;165(1):201-206.
doi: 10.1007/s00705-019-04465-w. Epub 2019 Nov 19.

Comparative thermostability analysis of zoonotic and human influenza virus A and B neuraminidase

Vasily A Evseenko  1 , Svetlana V Svyatchenko  2 , Natalia P Kolosova  2 , Valentina L Kovrizhkina  2 , Vasiliy Y Marchenko  2 , Aleksander G Durymanov  2 , Natalia I Goncharova  2 , Alexander B Ryzhikov  2
Affiliations
Comparative Study

Comparative thermostability analysis of zoonotic and human influenza virus A and B neuraminidase

Vasily A Evseenko et al. Arch Virol. 2020 Jan.

Abstract

Neuraminidase (NA) thermostability of influenza A and B viruses isolated from birds, swine and humans was measured to evaluate its variability associated with host body temperature. The highest 50% inactivation temperature (IT50) was observed with H3N8 avian influenza virus (74 °C), and the lowest IT50 was observed with the seasonal human H3N2 virus (45.5 °C). The IT50 values of A(H1N1)pdm09 viruses 56.4-58.5 °C were statistically higher than that of the prepandemic strain A/Solomon Islands/03/06 (52.5 °C). An analysis of Ca2+ binding sites revealed the correspondence of amino acid changes to NA thermostability. This study demonstrates that changes in NA thermostability correspond to differences in host body temperature.

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Conflict of interest statement

Evseenko V.A. declares that he has no conflict of interest. Kolosova N.P. declares that she has no conflict of interest. Svyatchenko S.V. declares that she has no conflict of interest. Kovrizhkina V.L. declares that she has no conflict of interest. Durymanov A.G. declares that he has no conflict of interest. Goncharova N.A. declares that she has no conflict of interest. Marchenko V.Y. declares that he has no conflict of interest. Ryzhikov A.B. declares that he has no conflict of interest.

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