Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Oct;85(19):7322-6.
doi: 10.1073/pnas.85.19.7322.

Characterization of the cDNA encoding a protein binding to the major histocompatibility complex class II Y box

D K Didier  1 , J Schiffenbauer, S L Woulfe, M Zacheis, B D Schwartz
Affiliations

Characterization of the cDNA encoding a protein binding to the major histocompatibility complex class II Y box

D K Didier et al. Proc Natl Acad Sci U S A. 1988 Oct.

Abstract

The expression of HLA class II genes is regulated by a series of cis-acting elements and trans-acting factors. Several cis-acting elements have been identified and have been termed the Z box, X box, Y box, octamer, and "TATA" box. The Y box contains an inverted CCAAT box. By probing a phage lambda gt11 library with double-stranded oligonucleotides, we have directly isolated a cDNA encoding a Y box-binding protein designated YB-1. YB-1 binding has an absolute requirement for the CCAAT box and relative specificity for the Y box. It has a Mr of 35,414, contains 18% basic residues, and contains putative nuclear localization signals. An inverse correlation of YB-1 and HLA-DR beta chain mRNA levels suggests that YB-1 is a negative regulatory factor.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
    1. Cell. 1988 Apr 8;53(1):11-24 - PubMed
    1. Biochemistry. 1979 Nov 27;18(24):5294-9 - PubMed
    1. Nucleic Acids Res. 1980 Jan 11;8(1):1-20 - PubMed
    1. Cell. 1983 Mar;32(3):695-706 - PubMed

Publication types

Associated data