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. 2019 Oct 22:13:2063-2069.
doi: 10.2147/OPTH.S215322. eCollection 2019.

Quantitative Comparison Of Microvascular Metrics On Three Optical Coherence Tomography Angiography Devices In Chorioretinal Disease

Yifan Lu #  1   2 , Jay C Wang #  1   2   3 , Rebecca Zeng  2 , Raviv Katz  2 , Demetrios G Vavvas  2   3 , Joan W Miller  2   3 , John B Miller  1   2   3
Affiliations

Quantitative Comparison Of Microvascular Metrics On Three Optical Coherence Tomography Angiography Devices In Chorioretinal Disease

Yifan Lu et al. Clin Ophthalmol. .

Abstract

Purpose: Optical coherence tomography angiography (OCT-A) has emerged as a novel tool for the non-invasive imaging and evaluation of the retinal microvasculature. There is little existing literature that compares OCT-A microvasculature metrics across different OCT-A devices in chorioretinal diseases. Herein, we examined these metrics on three available OCT-A platforms.

Patients and methods: All subjects were scanned on each of three OCT-A devices: Optovue Avanti Angiovue, Topcon DRI-OCT Triton Swept-Source OCT, and Zeiss Cirrus 5000-HD-OCT Angioplex. Two investigators independently measured foveal avascular zone (FAZ) area. Superficial capillary plexus (SCP) and deep capillary plexus (DCP) vessel densities (VD) were calculated from binarized images with ImageJ software. Image quality across devices was qualitatively compared. Interclass correlation coefficient (ICC), Bland-Altman analysis, repeated measures ANOVA, and post-hoc tests were performed for statistical analysis.

Results: Thirteen eyes of seven patients with chorioretinal diagnoses were reviewed. ICC for FAZ measurement was 0.95. There was no significant difference in FAZ area across the three devices (p= 0.792). There was a significant difference in the SCP VD between the OCT-A devices (Triton 0.344 ± 0.013, Angiovue 0.323 ± 0.013, Angioplex 0.367 ± 0.014, p < 0.05). Significantly greater DCP VD was observed with Angioplex (0.385 ± 0.010) in comparison to both Triton (0.331 ± 0.009) and Angiovue (0.341 ± 0.020). A comparison of image quality revealed that Angiovue gives the highest quality, followed by Angioplex and Triton.

Conclusion: Core macular microvasculature metrics are now readily accessible on a variety of available OCT-A devices. While the FAZ can be reliably measured across all three devices in this study, there were significant differences for the vessel density in both the SCP and DCP. As a result, clinicians should be careful when comparing microvasculature metrics across different devices when using patient data in multicenter research investigations and clinical trials.

Keywords: Angioplex; Angiovue; OCTA; Optovue; Triton; Zeiss.

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Conflict of interest statement

Dr Joan W Miller reports personal fees from Amgen, Inc., KalVista Pharmaceuticals, Ltd., Alcon Research Institute, Genentech/Roche, Bausch + Lomb, Sunovion, Valeant Pharmaceuticals/Massachusetts Eye and Ear, ONL Therapeutics, LLC, and grants from Lowy Medical Research Institute, Ltd., outside the submitted work. In addition, Dr Miller has patents US 5,798,349, US 6,225,303, US 6,610,679, CA 2,185,644, and CA 2,536,069 with royalties paid to Valeant Pharmaceuticals, a patent US 7,811,832 with royalties paid to ONL Therapeutics, a patent for Metabolomics for Biomarkers of AMD pending. Dr John B Miller reports personal fees from Heidelberg, Zeiss, Allergan, and Alcon, outside the submitted work. The authors report no other conflicts of interest in this work.

Figures

Figure 1
Figure 1
SCP images and segmentation using Optovue RTVue-XR, Topcon DRI-OCT Triton Swept-Source OCT, and Zeiss Cirrus 5000-HD-OCT Angioplex.
Figure 2
Figure 2
SCP images after binarization using the Niblack automated local thresholding method.
Figure 3
Figure 3
(A) Bland-Altman plot showed agreement between the two investigators for the SCP FAZ area measurements. (B–D) Bland-Altman plot showing the level of agreement between FAZ area measurements for devices. (B) Triton vs Angiovue. (C) Triton vs Angioplex. (D) Angiovue vs Angioplex.
None
See this image and copyright information in PMC

References

    1. Matsunaga DR, Yi JJ, De Koo LO, et al. Optical coherence tomography angiography of diabetic retinopathy in human subjects. Ophthalmic Surg Lasers Imaging Retina. 2015;46(8):796–805. doi:10.3928/23258160-20150909-03 - DOI - PubMed
    1. Spaide RF, Klancnik JM Jr, Cooney MJ. Retinal vascular layers imaged by fluorescein angiography and optical coherence tomography angiography. JAMA Ophthalmol. 2015;133(1):45–50. doi:10.1001/jamaophthalmol.2014.3616 - DOI - PubMed
    1. Munk MR, Giannakaki-Zimmermann H, Berger L, et al. OCT-angiography: A qualitative and quantitative comparison of 4 OCT-A devices. PLoS One. 2017;12(5):1–14. doi:10.1371/journal.pone.0177059 - DOI - PMC - PubMed
    1. Waheed NK, Moult EM, Fujimoto JG, et al. Optical coherence tomography angiography of dry age-related macular degeneration. Dev Ophthalmol. 2016;56:91–100. doi:10.1159/000442784 - DOI - PMC - PubMed
    1. Kashani AH, Lee SY, Moshfeghi A, et al. Optical coherence tomography angiography of retinal venous occlusion. Retina. 2015;35(11):2323–2331. doi:10.1097/IAE.0000000000000811 - DOI - PubMed