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. 2020 Mar;34(3):e4761.
doi: 10.1002/bmc.4761. Epub 2020 Jan 17.

Quantification of selected aroma compounds in e-cigarette products and toxicity evaluation in HUVEC/Tert2 cells

Jean-Christophe Noël  1   2 , Daniela Rainer  2 , Ronald Gstir  2 , Matthias Rainer  1 , Günther Bonn  1   2
Affiliations

Quantification of selected aroma compounds in e-cigarette products and toxicity evaluation in HUVEC/Tert2 cells

Jean-Christophe Noël et al. Biomed Chromatogr. 2020 Mar.

Abstract

During recent years, the number of consumers using so-called e-cigarettes, which are electrical devices to aerosolize a liquid consisting of propylene glycol, glycerol, optional nicotine and flavoring chemicals, has been increasing. Aromas vary from common flavors such as mint to more unusual flavors such as buttermilk or pepperoni pizza. Consumers today can buy e-concentrates that consist of propylene glycol and aroma to blend their own desired flavor at home. Little is known about the composition and concentration of various aroma molecules in the different e-liquids and e-concentrates. In addition, the process of EU-wide regulation is still ongoing. The aim of this research study was to identify and quantify possible undesirable aroma compounds in e-liquids and e-concentrates. Flavoring chemicals such as estragole, benzaldehyde and cinnamaldehyde were quantified. The measurements were carried out on a GC-MS system. The results show the presence of highly concentrated flavoring compounds and limonene oxide in lemon-flavored e-concentrates. In the final step, samples and single-aroma standards were tested for their toxicity to HUVEC/Tert2 cells, where some single-flavoring chemicals such as cinnamic aldehyde revealed significant toxic effects.

Keywords: GC-MS; benzaldehyde; cell studies; cinnamaldehyde; e-cigarette; estragole.

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References

REFERENCES

    1. The Flavor and Extract Manufacturing Association of the United States (2012). Respiratory Health and Safety in the Flavor Manufacturing Workplace. Washington, DC: FEMA.
    1. Aszyk, J., Woźniak, M. K., Kubica, P., Kot-Wasik, A., Namieśnik, J., & Wasik, A. (2017). Comprehensive determination of flavouring additives and nicotine in e-cigarette refill solutions. Part II: Gas-chromatography-mass spectrometry analysis. Journal of Chromatography A, 1517, 156-164. https://doi.org/10.1016/j.chroma.2017.08.057
    1. Behar, R. Z., Wang, Y., & Talbot, P. (2018). Comparing the cytotoxicity of electronic cigarette fluids, aerosols and solvents. Tobacco Control, 27(3), 325-333. https://doi.org/10.1136/tobaccocontrol-2016-053472
    1. Bundesagentur für Riskiobewertung (BFR) (2001). Gesundheitliche Bewertung von Duftölen, die Safrol, Methyleugenol oder Estragol enthalten.
    1. Chen, J. L. (2018). Steele TW and Stuckey DC Metabolic reduction of resazurin; Location within the cell for cytotoxicity assays. Biotechnology and Bioengineering, 115(2), 351-358. https://doi.org/10.1002/bit.26475

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