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. 2020 Jan:168:105783.
doi: 10.1016/j.mimet.2019.105783. Epub 2019 Nov 21.

Genotyping Neisseria gonorrhoeae gyrA and penA antimicrobial genes from remnant Neisseria gonorrhoeae positive Cepheid Xpert® clinical specimens - A feasibility study

S Qadir  1 , O Ellis  2 , E M Keizur  3 , C Stafylis  3 , R Cortado  4 , J D Klausner  5 ; Adolescent Trials Network (ATN) CARES Team
Affiliations

Genotyping Neisseria gonorrhoeae gyrA and penA antimicrobial genes from remnant Neisseria gonorrhoeae positive Cepheid Xpert® clinical specimens - A feasibility study

S Qadir et al. J Microbiol Methods. 2020 Jan.

Abstract

Neisseria gonorrhoeae (NG) has developed resistance to most antibiotics, making it increasingly difficult to treat. Previous studies have predicted antimicrobial NG susceptibility based on the antimicrobial gene target DNA gyrase subunit A (gyrA) codon serine 91 and the penicillin-binding protein 2 (penA) using Roche Cobas® and Hologic APTIMA™ clinical specimens. We studied whether similar methods could be used on remnant NG-positive Cepheid Xpert® specimens.

Keywords: Antibiotic resistance; Genotyping; Neisseria gonorrhoeae; gyrA; penA.

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