Influence of olive leaves extract on hepatorenal injury in streptozotocin diabetic rats

Abstract

Medicinal plants have always been an important source of new alternative effective compounds for human therapy. Currently, there are many of scientific evidences indicate that the medicinal plants contain a lot of hypoglycemic chemical compounds. The purpose of the present study was to determine the influence of olive leaves extract on hepatorenal injury in diabetic male rats. Experimental diabetes was induced by streptozotocin (STZ). The levels of serum glucose, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, gamma glutamyl transferase, total bilirubin, creatinine, blood urea nitrogen, uric acid and malondialdehyde were significantly increased, while the levels of serum superoxide dismutase, glutathione and catalase were statistically decreased in untreated diabetic rats. Moreover, the histopathological examination showed several alterations in the structure of liver and kidney in untreated diabetic rats. Treatments with low dose and high dose of olive leaves extract in diabetic rats showed remarkable reducing and protecting influences of physiological and histopathological alterations. Moreover, the highly treatment efficiency was noted in diabetic rats treated with high dose followed by low dose of olive leaves extract. Additionally, the results of this study proved that the antioxidant activities of olive leaves extract played a vital role against the hepatorenal injury induced by diabetes. Finally, this study indicates to the importance of the use of olive leaves extract as promising alternative and complementary therapeutic agent against diabetes and its complications.

Keywords: Blood; Diabetes; Kidney; Liver; Olive leaves; Rats; Streptozotocin.

Fig. 1

(A–F) The levels of glucose (A), ALT (B), AST (C), ALP (D), GGT (E) and total bilirubin (F) in serum from control, STZ, STZ plus LD of olive leaves extract, STZ plus HD of olive leaves extract, LD of olive leaves extract and HD of olive leaves extract treated rats.

Fig. 1

(A–F) The levels of glucose (A), ALT (B), AST (C), ALP (D), GGT (E) and total bilirubin (F) in serum from control, STZ, STZ plus LD of olive leaves extract, STZ plus HD of olive leaves extract, LD of olive leaves extract and HD of olive leaves extract treated rats.

Fig. 1

(A–F) The levels of glucose (A), ALT (B), AST (C), ALP (D), GGT (E) and total bilirubin (F) in serum from control, STZ, STZ plus LD of olive leaves extract, STZ plus HD of olive leaves extract, LD of olive leaves extract and HD of olive leaves extract treated rats.

Fig. 2

(A–C) The levels of creatinine (A), BUN (B) and uric acid (C) in serum from control, STZ, STZ plus LD of olive leaves extract, STZ plus HD of olive leaves extract, LD of olive leaves extract and HD of olive leaves extract treated rats.

Fig. 2

(A–C) The levels of creatinine (A), BUN (B) and uric acid (C) in serum from control, STZ, STZ plus LD of olive leaves extract, STZ plus HD of olive leaves extract, LD of olive leaves extract and HD of olive leaves extract treated rats.

Fig. 3

(A–F) Photomicrographs of liver sections in each group. (A) Control, (B) STZ, (C) STZ plus LD of olive leaves extract, (D) STZ plus HD of olive leaves extract, (E) LD of olive leaves extract and (F) HD of olive leaves extract (×200) treated rats. Original magnification ×200.

Fig. 4

(A–H) Photomicrographs of renal corpuscle in each group. (A) Control, (B, C and D) STZ, (E) STZ plus LD of olive leaves extract, (F) STZ plus HD of olive leaves extract, (G) LD of olive leaves extract and (H) HD of olive leaves extract treated rats. Original magnification ×1000.