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. 2019 Nov 21;9(12):1008.
doi: 10.3390/ani9121008.

Copy Number Variation of the CADM2 Gene and Its Association with Growth Traits in Yak

Fei Ge  1 , Congjun Jia  1 , Min Chu  1 , Chunnian Liang  1 , Ping Yan  1
Affiliations

Copy Number Variation of the CADM2 Gene and Its Association with Growth Traits in Yak

Fei Ge et al. Animals (Basel). .

Abstract

Copy number variation (CNV) is currently accepted as a common source of genetic variation. It is reported that CNVs may influence the resistance to disease and complex economic traits, such as residual feed intake, muscle formation, and fat deposition in livestock. Cell adhesion molecule 2 (CADM2) is expressed widely in the brain and adipose tissue and can regulate body weight through the central nervous system. Growth traits are important economic traits for animal selection. In this study, we aimed to explore the effect of CADM2 gene copy number variants on yak growth traits. Here, two CNVs in the CADM2 gene were investigated using the quantitative polymerase chain reaction (qPCR), and the association of the CNVs with growth traits in yak was analyzed using statistical methods by SPSS software. Differences were considered significant if the p value was < 0.05. Statistical analysis indicated significant association of CADM2-CNV2 with the body weight of the Chinese Ashidan yak. A significant effect of CNV2 (p < 0.05) was found on body weight at 6 months. In CNV2, the gain-type copy number variation exhibited greater performance than the other variants, with greater body weight observed at 6 months (p < 0.05). To the best of our knowledge, this is the first attempt to investigate the function of CADM2-CNVs and their association with growth traits in animals. This may be a useful candidate marker in marker-assisted selection of yaks.

Keywords: CADM2 gene; association analysis; copy number variation (CNV); growth traits; yak.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The two copy number variation regions of the CADM2 gene. The yellow boxes represent the coding regions and red boxes represent the CNVRs.
Figure 2
Figure 2
Amplification curves and melt peaks of the CADM2 and BTF3 genes.
Figure 3
Figure 3
Distribution of different copy number variation (CNV) types in CNV1 and CNV2 in the Ashidan Yak.
See this image and copyright information in PMC

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