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. 2020:2062:291-325.
doi: 10.1007/978-1-4939-9822-7_15.

Affinity Proteomic Analysis of the Human Exosome and Its Cofactor Complexes

Kinga Winczura  1 , Michal Domanski  2 , John LaCava  3   4
Affiliations

Affinity Proteomic Analysis of the Human Exosome and Its Cofactor Complexes

Kinga Winczura et al. Methods Mol Biol. 2020.

Erratum in

Abstract

In humans, the RNA exosome consists of an enzymatically inactive nine-subunit core, with ribonucleolytic activity contributed by additional components. Several cofactor complexes also interact with the exosome-these enable the recruitment of, and specify the activity upon, diverse substrates. Affinity capture coupled with mass spectrometry has proven to be an effective means to identify the compositions of RNA exosomes and their cofactor complexes: here, we describe a general experimental strategy for proteomic characterization of macromolecular complexes, applied to the exosome and an affiliated adapter protein, ZC3H18.

Keywords: Affinity capture; Affinity proteomics; Cofactors; Exosome; Mass spectrometry.

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