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. 2020:2089:179-189.
doi: 10.1007/978-1-0716-0163-1_12.

Enzymatic Bioautographic Methods

I Ayelen Ramallo  1 , Mario O Salazar  1 , Ricardo L E Furlan  2
Affiliations

Enzymatic Bioautographic Methods

I Ayelen Ramallo et al. Methods Mol Biol. 2020.

Abstract

Enzymatic bioautography enables the detection of enzyme inhibitors absorbed on a thin-layer chromatography plate. Therefore, it is an assay format that is particularly useful for the detection of inhibitors present in complex mixtures. The inhibition properties of compounds separated by thin-layer chromatography can be directly analyzed to produce an inhibition profile. Here, we describe the conditions to detect inhibitor of the enzymes xanthine oxidase and β-glucosidase immobilized on agar gel.

Keywords: Bioautographic method; Bioguided isolation; Control assay; Immobilization in gel; Xanthine oxidase; β-Glucosidase.

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