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. 2020 Mar;32(3):e13764.
doi: 10.1111/nmo.13764. Epub 2019 Nov 26.

microRNA-mRNA network model in patients with achalasia

Orazio Palmieri  1 , Tommaso Mazza  2 , Gabrio Bassotti  3 , Antonio Merla  1 , Salvatore Tolone  4 , Tommaso Biagini  2 , Antonello Cuttitta  5 , Fabrizio Bossa  1 , Giuseppina Martino  1 , Tiziana Latiano  1 , Giuseppe Corritore  1 , Domenica Gioffreda  1 , Orazio Palumbo  6 , Massimo Carella  6 , Anna Panza  1 , Angelo Andriulli  1 , Anna Latiano  1
Affiliations

microRNA-mRNA network model in patients with achalasia

Orazio Palmieri et al. Neurogastroenterol Motil. 2020 Mar.

Abstract

Background: Achalasia is a rare idiopathic disease with a complex etio-pathogenesis still unknown. This study aimed to identify microRNA (miRNA)-mRNA regulatory networks underlying achalasia.

Methods: The investigation was performed in tissue specimens from 11 patients and five controls using the microarray technology followed by an integrated bioinformatics analysis.

Key results: One hundred and six miRNAs were significantly up-regulated and 64 were down-regulated in achalasia patients. The expression of the most 10 differential expressed miRNAs (miR-122-5p, miR-133a-3p, miR-504-5p, miR-187-3p, miR-133b, miR-200c-3p, miR-375, miR-200b-5p, miR-200b-3p, and miR203a) was confirmed by droplet digital PCR in an independent cohort. The interactions between the significant miRNAs and their targets uncovered 14 miRNA-mRNA interacting pairs with experimentally predicted genes (ie, FN1, ROCK2, DPYSL2), and 35 pairs with not experimentally target genes (ie, SULF1, MRVI1, PRKG1); all genes were involved in immune cell trafficking, skeletal and muscular system development, nervous system development macro-processes.

Conclusion & inferences: The mRNA-miRNA regulatory networks described in this study provide new insights in the genetic background of the disease, suggesting further investigations in novel pathogenic mechanisms.

Keywords: achalasia; expression profile; mRNA; microRNA.

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References

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