Presence of thymosin-like factors in human thymic epithelium conditioned medium

Abstract

The objective of the present study was to determine whether or not thymosin and conditioned medium from human thymus epithelial cultures (HTECM) contain similar fractions, capable of inducing T-cell differentiation. Therefore we tested the ability of rabbit antisera to different thymosin fractions (thymosin fractions 5, 6 and alpha 1) of both bovine and human origin to block the stimulatory effect of HTECM on Con A and PHA response of mouse thymocytes. We also looked for reactivity of the antisera toward tissues of man, mouse and calf, and to tissue cultures of man and mouse. Anti-thymosin fraction 5 and 6, but not anti-thymosin-alpha 1, were found to inhibit the stimulatory effect of HTECM on both Con A and PHA responses. This cannot be attributed to cytostatic or cytotoxic effects of the antisera on thymocytes. No effect was seen when using antiserum to kidney fraction 5 or normal rabbit serum. In both tissue sections and tissue cultures of the different species tested, anti-thymosin reacts only with thymus epithelial cells. The reactivity is blocked by neutralizing the antisera with thymosin fraction 5 but not by kidney fraction 5. The reactivity is also strongly diminished by neutralizing the antisera with HTECM but not with supernatants of conditioned media from non-thymic tissues. The observations are highly suggestive for the presence of similar fractions in HTECM and thymosin, and that both are secreted by thymus epithelial cells.