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. 2019 Nov 27;24(23):4336.
doi: 10.3390/molecules24234336.

Effects of Different Colored LEDs on the Enhancement of Biologically Active Ingredients in Callus Cultures of Gynura procumbens (Lour.) Merr

Thang Tung Lian  1 , Myat Myat Moe  2 , Yong Ju Kim  1   3 , Keuk Soo Bang  1   3
Affiliations

Effects of Different Colored LEDs on the Enhancement of Biologically Active Ingredients in Callus Cultures of Gynura procumbens (Lour.) Merr

Thang Tung Lian et al. Molecules. .

Erratum in

Abstract

Conventional fluorescent lamps that are used in tissue culture are costly light sources, exhibiting excessive wavelength emission-bandwidth that must be replaced by alternative, less costly, and much lower power-consuming energy sources. The use of Light-Emitting Diodes (LEDs) is the best option due to their potential role as elicitors of secondary metabolite production in many plant models. Gynura procumbens (G. procumbens) is widely used for treating various diseases. Here, leaf explants were cultivated in MS medium that was supplemented with 0.5 mg/L of naphthaleneacetic acid (NAA) and 2.0 mg/L of benzylaminopurine (BAP) for 30 days under white, blue, and red LEDs. Secondary metabolites were analyzed by High Performance Liquid Chromatography (HPLC) and Liquid Chromatography-Mass Spectrometry (LC-MS). Blue LEDs elicited the highest antioxidant activity, total flavonoid, and phenolic content. Furthermore, the content of cyanidin-monoglucosides significantly increased under blue light.

Keywords: Gynura procumbens; HPLC; LC-MS; LEDs; callus; cyanidin-monoglucosides; elicitor.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Gynura procumbens callus after 30-day culture under different Light-Emitting Diode (LED) lights. (a) Dark, (b) Blue, (c) White, and (d) Red.
Figure 2
Figure 2
Effect of LEDs on callus culture for (a) DPPH free-radical savaging activity, (b) Total phenolic content, (c)Total flavonoid content, and (d) Total anthocyanin content of G. procumbens in callus culture. Values are means ± standard deviation (SD) from three replicates (n = 3). Ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test were performed, where p < 0.05 and p < 0.001 are represented as * and ***, respectively.
Figure 3
Figure 3
HPLC profile for the effect of LED lights for cyanidin-monoglucoside content in G. procumbens callus (a) peak of cyanidin-monoglucosides (b) standard curve (c) HPLC results (d) retention time and area of cyanidin-monoglucosides. Values are means ± standard deviation (SD) from three replicates (n = 3). Ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test were performed, where p < 0.001 are represented as ***.
Figure 4
Figure 4
LC-MS profile for the effect of LED lights for cyanidin-monoglucoside content in G. procumbens calli. (a) Calibration curve of cyanidin-monoglucoside, (b) peak of cyanidin-monoglucosides, (c) effect of LEDs on cyanidin-monoglucoside content. Values are means ± standard deviation (SD) from three replicates (n = 3). Ordinary one-way ANOVA followed by Dunnett’s multiple comparisons test were performed, where p < 0.05, p < 0.001, p < 0.0001, are represented as *, *** and ****, respectively.
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