Synthesis of sol-gel derived calcium silicate particles and development of a bioactive endodontic cement

Abstract

Objective: The aim of this study is to produce sol-gel derived calcium silicate particles (CS) and evaluate the influence of different concentration of calcium tungstate in the physical, chemical, mechanical and biological properties of developed cements.

Methods: Sol-gel route were used to synthesize calcium silicate particles that were characterized with x-ray difraction, Fourier transformed infrared spectroscopy, scanning electron microscopy, laser diffraction and nitrogen absorption. Cements were formulated with the addition of different concentrations of calcium tungstate (CaWO₄), resulting in four experimental groups according to the CS:CaWO₄ ratio: CS₁₀₀ (100:0), CS₉₀ (90:10), CS₈₀ (80:20), CS₇₀ (70:30). The setting time, radiopacity, compressive strength, pH, calcium release, cell proliferation and cell differentiation were used to characterize the cements.

Results: CS particles were succesfully sinthesized. The addition of CaWO₄ increased the radiopacity and did not influenced the setting time and the mechanical properties of cements. The pH of distilled water was increased for all groups and the CS₁₀₀ and CS₉₀ groups presented incresed calcium release. Reduced cell viability was found for CS₇₀ while CS₁₀₀ and CS₉₀ presented higher ALP activity and % of mineralized nodules after 21 days.

Significance: Sol-gel derived CS particles were sucssfully developed with potential to applied for the production of bioactive ceramic cements. The addition of 10% of CaWO₄ resulted in cements with adequate properties and bioactivity being an alternative for regenerative endodontic treatments.

Keywords: Calcium silicate; Calcium tungstate; Endodontic sealer; Regenerative endocontics.