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. 2020 Jan;8(1):e1046.
doi: 10.1002/mgg3.1046. Epub 2019 Nov 30.

Relationship between HLA-DPA1 genetic polymorphism and anembryonic pregnancy

Zhendong Wang  1 , Xiaolin Lu  2 , Xiuying Yao  3 , Xinli Liu  3 , Linlin Zhao  3 , Shaoyan Chang  2 , Ting Zhang  2 , Bo Niu  1   2 , Li Wang  2
Affiliations

Relationship between HLA-DPA1 genetic polymorphism and anembryonic pregnancy

Zhendong Wang et al. Mol Genet Genomic Med. 2020 Jan.

Abstract

Background: Human leukocyte antigen (HLA)-DP is an HLA class II molecule. Overexpression of HLA class II molecules in placental trophoblast cells may induce pregnancy loss. However, the association between HLA-DP and pregnancy loss remains unclear. HLA-DPA1 is an HLA-DP peptide chain. The objective of this study was to assess the association between HLA-DPA1 genetic polymorphism and anembryonic pregnancy, a type of early pregnancy loss, in the Chinese population.

Methods: A case-control study was designed to compare the frequencies of HLA-DPA1 gene polymorphisms in an anembryonic pregnancy group and a control group. Sixty-eight cases and 122 controls were recruited. Statistical analysis was performed to assess the correlation between single-nucleotide polymorphisms (SNPs) and anembryonic pregnancy susceptibility. MassARRAY high-throughput DNA analysis was used to analyze 19 HLA-DPA1 SNPs. To explore how HLA-DPA1 polymorphism could affect anembryonic pregnancy, HLA-DPA1 serum levels were analyzed by ELISA.

Results: Homozygous typing of rs1431403 (CC and TT) significantly increased the risk of anembryonic pregnancy in the case group (ORCC = 3.13, 95% CI: 1.50-6.53; ORTT = 2.96, 95% CI: 1.31-6.66; ORCC+TT = 3.06, 95% CI: 1.62-5.78). In samples with high HLA-DPA1 levels (≥1,500 pg/ml), the homozygous rs1431403 genotypes (nCC = 21, 43.8%; nTT = 20, 57.1%) were observed more frequently than were heterozygous genotypes.

Conclusion: HLA-DPA1 rs1431403 may be a risk factor for anembryonic pregnancy in the Chinese population. Homozygous rs1431403 genotypes (CC and TT) may increase the risk of anembryonic pregnancy by aberrantly increasing the HLA-DPA1 levels.

Keywords: HLA-DPA1; Immune Factors; Single-nucleotide polymorphism (SNP); anembryonic pregnancy; association study.

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Conflict of interest statement

We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work, there is no professional or other personal interest of any nature or kind in any product, service, and/or company that could be construed as influencing the position presented in, or the review of, the manuscript entitled, “Relationship between HLA‐DPA1 Genetic Polymorphism and Anembryonic Pregnancy”.

Figures

Figure 1
Figure 1
Human leukocyte antigen‐DPA1 (HLA‐DPA1) expression in anembryonic pregnancy and control groups. The ordinate is the concentration of HLA‐DPA1 (GRCh37.p13), which was logarithmically transformed. Case and control group data are presented. p = .125, as calculated by the Student's t test
Figure 2
Figure 2
Human leukocyte antigen‐DPA1 (HLA‐DPA1) expression in samples based on rs1431403 genotype. The ordinate is the HLA‐DPA1 (GRCh37.p13) concentration, which was logarithmically transformed. Circular represented samples with their genotypes of the rs1431403 CT, CC, and TT genotypes are presented. p = .085, as calculated by analysis of variance
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