Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Mar;14(2):142-149.
doi: 10.1111/irv.12701. Epub 2019 Dec 1.

Rapid detection of respiratory organisms with FilmArray respiratory panel and its impact on clinical decisions in Shanghai, China, 2016-2018

Yiyi Qian  1 , Jingwen Ai  1 , Jing Wu  1 , Shenglei Yu  1 , Peng Cui  1 , Yan Gao  1 , Jialin Jin  1 , Xinhua Weng  1 , Wenhong Zhang  1   2   3   4
Affiliations

Rapid detection of respiratory organisms with FilmArray respiratory panel and its impact on clinical decisions in Shanghai, China, 2016-2018

Yiyi Qian et al. Influenza Other Respir Viruses. 2020 Mar.

Abstract

Background: In this study, we evaluated the diagnostic potential and clinical impact of an automated multiplex PCR platform (the FilmArray Respiratory Panel; FA-RP), specially designed for pathogen detection in respiratory tract infections in adults with unexplained pneumonia (UP).

Methods: A total of 112 UP patients in Shanghai, China, were enrolled prospectively and assessed using the FA-RP from October 2016 to March 2018. We examined the test results and their influence on clinical decisions. Furthermore, as a control group, we retrospectively obtained the clinical data of 70 UP patients between October 2014 and March 2016 (before the FA-RP was available). The two patient groups were compared with respect to factors, including general antimicrobial use and defined daily dose (DDD) numbers.

Results: Between October 2016 and March 2018, the positive rate obtained using FA-RP for UP was 76.8%. The primary pathogens in adults with UP were Influenza A/B (47.3%, 53/112). Compared with the patients before FA-RP was available, patients who underwent FA-RP testing had higher rates of antiviral drug use and antibiotic de-escalation during clinical treatment. FA-RP significantly decreased the total DDDs of antibiotic or antifungal drugs DDDs by 7 days after admission (10.6 ± 2.5 vs 14.1 ± 8.8, P < .01).

Conclusions: The FA-RP is a rapid and sensitive nucleic acid amplification test method for UP diagnosis in adults. The application of FA-RP may lead to a more accurately targeted antimicrobial treatment and reduced use of antibiotic/antifungal drugs.

Keywords: FilmArray respiratory panel; antibiotic use; clinical decision; respiratory virus; unexplained pneumonia.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Overview of Patient Enrollment Workflow. A total of 112 patients selected prospectively were submitted to FA‐RP analyses and are referred to as the “FA‐RP group.” Those who were not lost to follow‐up and had complete data are referred to as the “intervention group.” Comparison was made between it and the historical control group
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Qian Y‐H, Su J, Shi P, et al. Attempted early detection of influenza A (H1N1) pandemic with surveillance data of influenza‐like illness and unexplained pneumonia. Influenza Other Respir Viruses. 2011;5(6):e479‐e486. - PMC - PubMed
    1. Gavin PJ, Thomson RB. Review of rapid diagnostic tests for influenza. Clin Appl Immunol Rev. 2004;4(3):151‐172.
    1. Zumla A, Al‐Tawfiq JA, Enne VI, et al. Rapid point of care diagnostic tests for viral and bacterial respiratory tract infections—needs, advances, and future prospects. Lancet Infect Dis. 2014;14(11):1123‐1135. - PMC - PubMed
    1. Poritz MA, Blaschke AJ, Byington CL, et al. FilmArray, an automated nested multiplex PCR system for multi‐pathogen detection: development and application to respiratory tract infection. PLoS ONE. 2011;6(10):e26047. - PMC - PubMed
    1. Branche AR, Walsh EE, Formica MA, Falsey AR. Detection of respiratory viruses in sputum from adults by use of automated multiplex PCR. J Clin Microbiol. 2014;52(10):3590‐3596. - PMC - PubMed

Publication types

MeSH terms