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. 2019 Nov 27:16:81.
doi: 10.1186/s12986-019-0401-4. eCollection 2019.

The effect of western diet on mice brain lipid composition

Alicja Pakiet  1 , Agnieszka Jakubiak  2 , Aleksandra Czumaj  3 , Tomasz Sledzinski  3 , Adriana Mika  1   3
Affiliations

The effect of western diet on mice brain lipid composition

Alicja Pakiet et al. Nutr Metab (Lond). .

Erratum in

Abstract

Background: The appropriate fatty acids composition of brain lipids is critical for functioning of this organ. The alterations of brain fatty acids composition may lead to neurological and neurodegenerative diseases.

Methods: The aim of this work was to evaluate the effect of western diet containing high fat content on fatty acid composition of brain lipids. In this study we used mice fed high fat diet (HFD) for 19 weeks. Brain lipids were separated by SPE extraction and fatty acid composition in chow, mice serum, brain and other tissues was analyzed by GC-MS method.

Results: The body weight and adipose tissue weigh of mice after HFD increased significantly. The concentrations of most of fatty acids in serum of mice after HFD increased, due to their higher delivery from food. Unexpectedly the serum eicosapentaenoic acid (EPA) concentration was lower in mice after HFD than in controls. Also the brain, and other tissue EPA content was lower. Among studied groups of brain lipids EPA was significantly decreased in phospholipids and sphingolipids.

Conclusions: Considering important role of brain EPA including maintaining of appropriate composition of cell membrane lipids and anti-inflammatory properties we conclude that decrease of brain EPA after western diet may result in impaired brain function.

Keywords: Docosahexaenoic acid; Eicosapentaenoic acid; High-fat diet; Mice model; Phospholipids; Polyunsaturated fatty acids; Sphingolipids; brain; obesity.

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Conflict of interest statement

Competing interestsThe authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
The body weight of mice with obesity induced by high fat diet and control mice. HFD – high fat diet; SD – standard diet. p-value: * < 0.05; # < 0.001
Fig. 2
Fig. 2
The level of total fatty acids in serum of SD and HFD mice. HFD – high fat diet; SD – standard diet. p-value: * < 0.05
Fig. 3
Fig. 3
Composition of polar lipids in mice brain obtained with Kaluzny et al. [27] method. AG – acylglycerols; HFD – high fat diet; PL – polar lipids; SD – standard diet; FFA – free fatty acids. p-value from t-test: # < 0.001
Fig. 4
Fig. 4
Composition of polar lipids in mice brain obtained with Bodennec et al. [28] method. CER – ceramides; GSPL – glycosphingolipids; HFD – high-fat diet; SD – standard diet; SM – sphingomyelins. p-value from t-test: * < 0.05
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