Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Dec 3;187(1):1.
doi: 10.1007/s00604-019-3921-8.

An impedimetric biosensor based on electrophoretically assembled ZnO nanorods and carboxylated graphene nanoflakes on an indium tin oxide electrode for detection of the DNA of Escherichia coli O157:H7

Nandita Jaiswal  1 , Chandra Mouli Pandey  2 , Shipra Solanki  3 , Ida Tiwari  4 , Bansi Dhar Malhotra  3
Affiliations

An impedimetric biosensor based on electrophoretically assembled ZnO nanorods and carboxylated graphene nanoflakes on an indium tin oxide electrode for detection of the DNA of Escherichia coli O157:H7

Nandita Jaiswal et al. Mikrochim Acta. .

Abstract

Aminopropyltrimethoxysilane (APTMS)-functionalized zinc oxide (ZnO) nanorods and carboxylated graphene nanoflakes (c-GNF) were used in a composite that was electrophoretically deposited on an indium tin oxide (ITO) coated glass substrate. The modified ITO electrodes were characterized using various microscopic and spectroscopic techniques which confirm the deposition of the APTMS-ZnO/c-GNF composite. The electrodes have been used for the covalent immobilization of an Escherichia coli O157:H7 (E. coli)-specific DNA prob. Impedimetric studies revealed that the gene sensor displays linear response in a wide range of target DNA concentration (10-16 M to 10-6 M) with a detection limit of 0.1 fM. The studies on the cross-reactivity to other water-borne pathogens show that the bioelectrode is highly specific. Graphical abstractSchematic illustration for fabrication of nucleic acid biosensor for E. coli DNA detection using an ITO electrode modified with siloxane-functionalized zinc oxide (ZnO) nanorods and carboxylated graphene nanoflakes (c-GNFs).

Keywords: Biosensor; Electrophoretic deposition; Escherichia coli; Graphene nanoflakes; Zinc oxide.

PubMed Disclaimer

References

    1. Analyst. 2005 Apr;130(4):421-6 - PubMed
    1. Nanoscale. 2013 Jun 7;5(11):4758-62 - PubMed
    1. Chem Soc Rev. 2013 Jun 21;42(12):5425-38 - PubMed
    1. Talanta. 2017 Jan 1;162:511-522 - PubMed
    1. Talanta. 2011 Aug 15;85(2):943-9 - PubMed

Publication types