Higher frequency of vertebrate-infecting viruses in the gut of infants born to mothers with type 1 diabetes

Ki Wook Kim¹, Digby W Allen¹, Thomas Briese², Jennifer J Couper³, Simon C Barry³, Peter G Colman⁴, Andrew M Cotterill⁵, Elizabeth A Davis⁶, Lynne C Giles⁷, Leonard C Harrison⁸, Mark Harris⁵, Aveni Haynes⁶, Jessica L Horton¹, Sonia R Isaacs¹, Komal Jain², Walter I Lipkin², Kelly McGorm³, Grant Morahan⁹, Claire Morbey¹⁰, Ignatius C N Pang¹¹, Anthony T Papenfuss⁸, Megan A S Penno³, Richard O Sinnott¹², Georgia Soldatos¹³, Rebecca L Thomson³, Peter Vuillermin¹⁴, John M Wentworth⁸, Marc R Wilkins¹¹, William D Rawlinson^{1

15}, Maria E Craig^{1

16}; ENDIA STUDY GROUP

Affiliations

¹ School of Women's and Children's Health, University of New South Wales, Sydney, New South Wales, Australia.
² Center for Infection and Immunity and Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, New York.
³ Robinson Research Institute and Adelaide Medical School, University of Adelaide, Adelaide, South Australia, Australia.
⁴ Department of Diabetes and Endocrinology, The Royal Melbourne Hospital Victoria, Melbourne, Victoria, Australia.
⁵ Department of Endocrinology, Queensland Children's Hospital, South Brisbane, Queensland, Australia.
⁶ Telethon Kids Institute, The University of Western Australia, Perth, Western Australia, Australia.
⁷ School of Public Health, University of Adelaide, Adelaide, South Australia, Australia.
⁸ Walter and Eliza Hall Institute and Royal Melbourne Hospital, Melbourne, Victoria, Australia.
⁹ Centre for Diabetes Research, Harry Perkins Institute of Medical Research, Perth, Western Australia, Australia.
¹⁰ Hunter Diabetes Centre, Newcastle, New South Wales, Australia.
¹¹ School of Biotechnology and Biomolecular Science, University of New South Wales, Sydney, New South Wales, Australia.
¹² Department of Computing and Information Systems, University of Melbourne, Melbourne, Victoria, Australia.
¹³ Monash Centre for Health Research and Implementation, School of Public Health and Preventive Medicine, Monash University, Melbourne, Victoria, Australia.
¹⁴ School of Medicine, Deakin University, Geelong, Victoria, Australia.
¹⁵ Serology and Virology Division, SEALS Microbiology, Prince of Wales Hospital, Sydney, New South Wales, Australia.
¹⁶ Institute of Endocrinology and Diabetes, The Children's Hospital at Westmead, Sydney, New South Wales, Australia.

PMID: 31800147
DOI: 10.1111/pedi.12952

Higher frequency of vertebrate-infecting viruses in the gut of infants born to mothers with type 1 diabetes

Ki Wook Kim et al. Pediatr Diabetes. 2020 Mar.

. 2020 Mar;21(2):271-279.

doi: 10.1111/pedi.12952. Epub 2020 Jan 7.

Authors

Affiliations

¹ School of Women's and Children's Health, University of New South Wales, Sydney, New South Wales, Australia.
² Center for Infection and Immunity and Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, New York.
³ Robinson Research Institute and Adelaide Medical School, University of Adelaide, Adelaide, South Australia, Australia.
⁴ Department of Diabetes and Endocrinology, The Royal Melbourne Hospital Victoria, Melbourne, Victoria, Australia.
⁵ Department of Endocrinology, Queensland Children's Hospital, South Brisbane, Queensland, Australia.
⁶ Telethon Kids Institute, The University of Western Australia, Perth, Western Australia, Australia.
⁷ School of Public Health, University of Adelaide, Adelaide, South Australia, Australia.
⁸ Walter and Eliza Hall Institute and Royal Melbourne Hospital, Melbourne, Victoria, Australia.
⁹ Centre for Diabetes Research, Harry Perkins Institute of Medical Research, Perth, Western Australia, Australia.
¹⁰ Hunter Diabetes Centre, Newcastle, New South Wales, Australia.
¹¹ School of Biotechnology and Biomolecular Science, University of New South Wales, Sydney, New South Wales, Australia.
¹² Department of Computing and Information Systems, University of Melbourne, Melbourne, Victoria, Australia.
¹³ Monash Centre for Health Research and Implementation, School of Public Health and Preventive Medicine, Monash University, Melbourne, Victoria, Australia.
¹⁴ School of Medicine, Deakin University, Geelong, Victoria, Australia.
¹⁵ Serology and Virology Division, SEALS Microbiology, Prince of Wales Hospital, Sydney, New South Wales, Australia.
¹⁶ Institute of Endocrinology and Diabetes, The Children's Hospital at Westmead, Sydney, New South Wales, Australia.

PMID: 31800147
DOI: 10.1111/pedi.12952

Abstract

Background: Microbial exposures in utero and early life shape the infant microbiome, which can profoundly impact on health. Compared to the bacterial microbiome, very little is known about the virome. We set out to characterize longitudinal changes in the gut virome of healthy infants born to mothers with or without type 1 diabetes using comprehensive virome capture sequencing.

Methods: Healthy infants were selected from Environmental Determinants of Islet Autoimmunity (ENDIA), a prospective cohort of Australian children with a first-degree relative with type 1 diabetes, followed from pregnancy. Fecal specimens were collected three-monthly in the first year of life.

Results: Among 25 infants (44% born to mothers with type 1 diabetes) at least one virus was detected in 65% (65/100) of samples and 96% (24/25) of infants during the first year of life. In total, 26 genera of viruses were identified and >150 viruses were differentially abundant between the gut of infants with a mother with type 1 diabetes vs without. Positivity for any virus was associated with maternal type 1 diabetes and older infant age. Enterovirus was associated with older infant age and maternal smoking.

Conclusions: We demonstrate a distinct gut virome profile in infants of mothers with type 1 diabetes, which may influence health outcomes later in life. Higher prevalence and greater number of viruses observed compared to previous studies suggests significant underrepresentation in existing virome datasets, arising most likely from less sensitive techniques used in data acquisition.

Keywords: enterovirus; gut; longitudinal; type 1 diabetes; virome.

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References

REFERENCES

1. Ihekweazu FD, Versalovic J. Development of the pediatric gut microbiome: impact on health and disease. Am J Med Sci. 2018;356:413-423.
1. Lim ES, Wang D, Holtz LR. The bacterial microbiome and virome milestones of infant development. Trends Microbiol. 2016;24:801-810.
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Higher frequency of vertebrate-infecting viruses in the gut of infants born to mothers with type 1 diabetes

Affiliations

Higher frequency of vertebrate-infecting viruses in the gut of infants born to mothers with type 1 diabetes

Authors

Affiliations

Abstract

References

REFERENCES

Publication types

MeSH terms

Associated data

Grants and funding

LinkOut - more resources

Full Text Sources

Medical