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. 2019 Dec;11(12):789-797.
doi: 10.14740/jocmr3989. Epub 2019 Nov 24.

Plasma Levels of Myocardial MicroRNA-133a Increase by Intraoperative Cytokine Hemoadsorption in the Complex Cardiovascular Operation

Robert Wagner  1   2 , Premysl Soucek  3   2 , Jiri Ondrasek  4 , Petr Fila  4 , Jan Sterba  4 , Hana Spacilova  5 , Alzbeta Michalcikova  6 , Tomas Freiberger  3 , Petr Nemec  4
Affiliations

Plasma Levels of Myocardial MicroRNA-133a Increase by Intraoperative Cytokine Hemoadsorption in the Complex Cardiovascular Operation

Robert Wagner et al. J Clin Med Res. 2019 Dec.

Abstract

Background: Complex cardiovascular procedures may initiate a systemic inflammatory response syndrome (SIRS) with a massive cytokine release, which is involved in postoperative myocardial injury. Intraoperative cytokine hemoadsorption (HA) mitigates the inflammatory response. Micro ribonucleic acids (miRNAs) are emerging as a marker of myocardial injury.

Methods: This study evaluated if intraoperative cytokine reduction by HA modulates SIRS and affects myocardial injury as measured by miRNA-126, 223 and miRNA-1, 133a, respectively. Twenty-eight patients were assigned into HA (n = 15) and control (C) (n = 13) groups. HA was performed by integrating CytoSorb™ into the extracorporeal circuit.

Results: MiRNA-133a plasma levels were increased postoperatively in both groups but were much higher in the HA group than in the C group at 3 h (P = 0.037) and 18 h (P = 0.017) after reperfusion. MiRNA-1 and miRNA-223 plasma levels were significantly increased postoperatively, but did not differ between groups. The vascular miRNA-126 was not affected.

Conclusion: Intraoperative cytokine HA in cardiovascular operations increased the plasma levels of miRNA-133a, suggesting higher myocardial injury.

Keywords: Cardiac operations; Cytokines; Hemoadsorption; MicroRNA; Myocardial injury; Systemic inflammatory response syndrome.

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Conflict of interest statement

None to declare.

Figures

Figure 1
Figure 1
Flow diagram.
Figure 2
Figure 2
Changes of myogenic miRNAs after surgery. Con: control group; HA: hemoadsorption group. Time points: T1: after induction of anesthesia, T2: 3 h (after skin incision), T3: 6 h (after ICU arrival), T4: 18 h (the first postoperative day, 5 o’clock a.m.) and T5: 42 (the second postoperative day, 5 o’clock a.m.) after aortic declamping. Label a: The plasma levels of miRNA-133a were increased postoperatively in both groups but increased much more in the HA group at T2 (Con 2 versus HA 2) and T4 (Con 4 versus HA 4). Label b: The plasma levels of miRNA-1 were increased postoperatively in both groups, but without the difference between groups.
See this image and copyright information in PMC

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