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. 2020 May;52(3):1479-1485.
doi: 10.1007/s11250-019-02151-2. Epub 2019 Dec 5.

More insights about the interfering effect of Mycobacterium avium subsp. paratuberculosis (MAP) infection on Mycobacterium bovis (M. bovis) detection in dairy cattle

E Raffo  1   2 , P Steuer  1   2 , C Tomckowiack  1   2 , C Tejeda  1   2 , B Collado  1   2 , M Salgado  3
Affiliations

More insights about the interfering effect of Mycobacterium avium subsp. paratuberculosis (MAP) infection on Mycobacterium bovis (M. bovis) detection in dairy cattle

E Raffo et al. Trop Anim Health Prod. 2020 May.

Abstract

The estimated herd and within herd Mycobacterium bovis (M. bovis) infection prevalence in the southern Chile regions are 0.3 and 0.67%, respectively. However, higher rates of infection still remain in some herds. In parallel, it is well established that a big proportion of cattle herds are infected with Mycobacterium avium subsp. paratuberculosis (MAP), which has been also associated with a clear interference effect on M. bovis diagnosis. The present study aims to provide more insights about the diagnostic interference for Mycobacterium bovis detection due to co-infection with MAP. To better understand the dynamics of this identified interference, the effect of MAP genotype present, as well as MAP faecal shedding values (as proxy of the infection progression), for each of the CFT results was compared. No relationship was observed between MAP genotype with any type of differential response to the diagnostic tests of M. bovis infection. However, MAP shedding values in animals with positive CFT diagnostic results for M. bovis infection was significantly lower than animals with a negative CFT result, observing that as the MAP shedding load raises, the response to the bovine tuberculin test tends to be negative. The findings reported in this study allows to interpret that one of the causes of the prolonged elimination of M. bovis infection from some cattle herds may be due in part to the advanced MAP infection status in co-infected individuals affecting the outcome of screening in-vivo diagnostic techniques such as CFT. These false negative animals that show negative results to M. bovis detection tests, may maintain the infection at herd level and spread the pathogen to healthy individuals.

Keywords: Bovine tuberculosis; CFT; Interference; M. bovis; MAP; PCR; Paratuberculosis.

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References

    1. Alvarez, J., de Juan, L., Bezos, J., Romero, B., Sáez, J.L., Marqués, S., Domínguez, C., Mínguez, O., Fernández-Mardomingo, B., Mateos, A., Domínguez, L., Aranaz, A., 2009. Effect of paratuberculosis on the diagnosis of bovine tuberculosis in a cattle herd with a mixed infection using interferon-gamma detection assay. Veterinary microbiology 135, 389–393. - DOI
    1. Alvarez, J., de Juan, L., Bezos, J., Romero, B., Saez, J.L., Reviriego Gordejo, F.J., Briones, V., Moreno, M.A., Mateos, A., Dominguez, L., Aranaz, A., 2008. Interference of paratuberculosis with the diagnosis of tuberculosis in a goat flock with a natural mixed infection. Veterinary microbiology 128, 72–80. - DOI
    1. Allix, C., Walravens, K., Saegerman, C., Godfroid, J., Supply, P., Fauville-Dufaux, M., 2006. Evaluation of the epidemiological relevance of variable-number tandem-repeat genotyping of Mycobacterium bovis and comparison of the method with IS6110 restriction fragment length polymorphism analysis and spoligotyping. Journal of clinical microbiology 44, 1951–1962. - DOI
    1. Amonsin, A., Li, L.L., Zhang, Q., Bannantine, J.P., Motiwala, A.S., Sreevatsan, S., Kapur, V., 2004. Multilocus short sequence repeat sequencing approach for differentiating among Mycobacterium avium subsp. paratuberculosis strains. Journal of clinical microbiology 42, 1694–1702. - DOI
    1. Aranaz, A., De Juan, L., Bezos, J., Alvarez, J., Romero, B., Lozano, F., Paramio, J.L., Lopez-Sanchez, J., Mateos, A., Dominguez, L., 2006. Assessment of diagnostic tools for eradication of bovine tuberculosis in cattle co-infected with Mycobacterium bovis and M. avium subsp. paratuberculosis. Veterinary research 37, 593–606. - DOI

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