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. 2019 Nov 19;4(23):20118-20128.
doi: 10.1021/acsomega.9b00655. eCollection 2019 Dec 3.

In Vitro and in Vivo Studies of pH-Sensitive GHK-Cu-Incorporated Polyaspartic and Polyacrylic Acid Superabsorbent Polymer

Shilpa Sharma  1   2 , Mohammad Faiyaz Anwar  2 , Amit Dinda  2 , Maneesh Singhal  2 , Amita Malik  1
Affiliations

In Vitro and in Vivo Studies of pH-Sensitive GHK-Cu-Incorporated Polyaspartic and Polyacrylic Acid Superabsorbent Polymer

Shilpa Sharma et al. ACS Omega. .

Abstract

The main aim of this study was to evaluate the in vitro and in vivo efficiency of the polyaspartic acid- and acrylic acid-based superabsorbent polymer. The synthesized polymer was first investigated to check the blood compatibility by protein adsorption and blood clotting tests. Further, the GHK-Cu peptide was incorporated within the polymer and release studies were performed to evaluate the drug-delivery efficiency of the superabsorbent polymer. The polymer with best peptide release results were further used for in vivo analysis for wound healing. The healing efficiency of polymer with and without peptide was analyzed using wound closure, biochemical assay, histopathological, and toxicity studies.

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Conflict of interest statement

The authors declare the following competing financial interest(s): The financial funding was provided by University of Grant Commision, New Delhi, India (Grant No: F.No. 42-255/2013 (SR)).

Figures

Figure 1
Figure 1
(a) Blood clotting index and (b) protein adsorption of polymer samples.
Figure 2
Figure 2
Peptide release from (a) EGDMA- and (b) TMPTA-based superabsorbent polymer.
Figure 3
Figure 3
(a) FTIR and (b) EDX spectra of peptide-encapsulated EGDMA-based polymer.
Figure 4
Figure 4
(a) FTIR and (b) EDX spectra of peptide-encapsulated TMPTA-based polymer.
Figure 5
Figure 5
Wound closure on 0th, 8th, and 15th days using polymer with and without encapsulated GHK-Cu (n = 6) (p < 0.05).
Figure 6
Figure 6
Granulation activity of wound by using with (A″) and without (A) peptide-encapsulated polymer.
Figure 7
Figure 7
Antioxidant activity of wound by using with and without peptide-encapsulated polymer at 8th and 15th days.
Figure 8
Figure 8
Histopathological microphotographs of different groups on 8th day: (a) control (group I); (b) burned (group II); and (c) PASP:AA-treated groups (group III-A). (d) Polymer with GHK-Cu (group IV-A″) and on 15th day, (e) burned (group II), (f) PASP:AA (group III-A), and (g) polymer with GHK-Cu (group IV-A″).
Figure 9
Figure 9
Toxicity levels found in rat after using polymeric material for wound healing.
See this image and copyright information in PMC

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