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. 2019 Dec 12;8(50):e01314-19.
doi: 10.1128/MRA.01314-19.

Draft Whole-Genome Sequences of Two Western European Borrelia miyamotoi Isolates

Konstantin V Kuleshov  1   2 , Dieuwertje Hoornstra  3 , Hein Sprong  4 , Alexander E Platonov  5 , Joppe W Hovius  3
Affiliations

Draft Whole-Genome Sequences of Two Western European Borrelia miyamotoi Isolates

Konstantin V Kuleshov et al. Microbiol Resour Announc. .

Abstract

We report the draft whole-genome sequences of two Borrelia miyamotoi strains isolated in The Netherlands. Using next-generation sequencing, we determined the complete sequence of the chromosomes and several plasmids. The two strains show a genotype typical of European strains, distinct from the genomes of strains from Asia or the United States.

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Figures

FIG 1
FIG 1
Concatenated core genes (n = 230) among 39 Borrelia genomes, including 19 Borrelia miyamotoi genomes, were generated through Roary v3.11.2 with the “-i 70” option (minimal identity percentage for BLASTP). A maximum likelihood (ML) phylogenetic tree was constructed using the RAxML software, employing a nucleotide substitution model with a gamma distribution of various positions (GTR + Γ) and the “-f a” option for rapid bootstrap analysis and to search for the best-scoring ML tree. The resulting tree was midpoint rooted (http://tree.bio.ed.ac.uk/software/figtree/). Cut long branches indicated by dashed lines. Scale bar indicates substitution rates.
See this image and copyright information in PMC

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