WNT gene polymorphisms and predisposition to apical periodontitis

Abstract

Single nucleotide polymorphisms (SNPs) in WNT genes may impact gene/protein function and contribute to individual predisposition to apical periodontitis (AP). Here, we investigated the association of SNPs in/nearby WNT3, WNT3A, WNT5A, WNT8A, WNT9B and WNT11 genes with AP using a case-control dataset. Cases were defined as individuals with deep caries and AP (n = 188); controls had deep caries and no AP (n = 230). Genotyping was performed using Taqman chemistry in real time PCR. Data analyses was performed using Fisher Exact tests assuming a Bonferroni correction threshold value of 0.005. Single-SNP association analysis revealed a trend for association with WNT3 rs9890413 genotypes (P = 0.009) under a dominant model and allelic association for WNT3A rs1745420 (P = 0.009). Haplotypes involving WNT3-WNT9B-WNT3A alleles were also significantly associated with AP (P ≤ 0.003). Luciferase reporter assays showed higher transcriptional activity (1.4-fold) with the alternate G allele in rs1745420. Expression of WNT3, WNT3A and WNT5A in AP tissues was significantly higher than in control tissues, and inversely correlated with the expression of SERPINB1, COL1A1 and TIMP1 (P < 0.05). Our results suggest that WNT genes have a role in modulating AP and polymorphisms in these genes may increase susceptibility to AP.

Figure 1

Results of luciferase reporter gene assays for WNT3A rs1745420. Allele-specific transcriptional activities are shown for ancestral (ANC) and (ALT) alleles.

Figure 2

Expression of WNT3, WNT3A, WNT5A and WNT9B mRNA in apical periodontitis (AP) and control tissues. Top, mRNA expression levels in AP lesions and controls. Bottom, mRNA expression levels in active and inactive AP lesions. *Indicates P ≤ 0.05.

Figure 3

Correlation analysis between expression of WNT3, WNT3A, WNT5A and WNT9B with SERPINB1, TIMP1 and COL1A1 in AP lesions. *Indicates P ≤ 0.05.