Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Dec 12;9(1):18994.
doi: 10.1038/s41598-019-55265-w.

Novel Bacteria-Immobilized Cellulose Acetate/Poly(ethylene oxide) Nanofibrous Membrane for Wastewater Treatment

Doaa Zamel  1   2 , Ahmed H Hassanin  3   4 , Rania Ellethy  5 , Gamal Singer  5 , Ahmed Abdelmoneim  3   6
Affiliations

Novel Bacteria-Immobilized Cellulose Acetate/Poly(ethylene oxide) Nanofibrous Membrane for Wastewater Treatment

Doaa Zamel et al. Sci Rep. .

Abstract

In this study, electrospun cellulose acetate - poly(ethylene oxide) nanofibrous membrane was found to be unique in immobilizing bacterial cells. Here, removal of methylene blue in aqueous media was achieved by using isolated species of bacteria (Bacillus paramycoides) from industrial wastewater and immobilized on cellulose acetate- poly(ethylene oxide) nanofibers using DMSO as a solvent. The decolorization time was varied from 0 to 72 h, different dye concentrations from 20 to 200 mg/L and bacterial cells count was investigated to achieve the maximum MB removal by bacteria-immobilized CA/PEO nanofibrous membrane. The effective dye decolorization was achieved within 48 h and MB removal % was around 93%. Furthermore, reusability of the bacteria-immobilized CA/PEO nanofibrous membrane was tested. It was found that after the 4th usage, 44% of the dye decolorization capacity still could be achieved. These results are promising and suggest that bacteria-immobilized CA/PEO nanofibrous membrane could be economically feasible and eco-friendly when used in MB removal from industrial wastewater. Combination of both adsorption and biodegradation methods was found to be effective in MB removal from aqueous media.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Representative SEM images of cellulose acetate- poly(ethylene oxide) nanofibers at different concentrations and fiber diameter distribution (A) 12; (B) 15 and (C) 18 wt%.
Figure 2
Figure 2
SEM images of Bacteria-immobilized CA/PEO nanofibrous membrane and fiber diameter distribution; (A) at low magnification, (B) at high magnification and (C) fiber diameter distribution.
Figure 3
Figure 3
Comparison between MB removal % achieved by CA/PEO nanofibers, bacteria-immobilized CA/PEO nanofibrous membrane and free bacteria at different time intervals.
Figure 4
Figure 4
The effect of different dye concentrations on the decolorization yield after 48 h incubation period.
Figure 5
Figure 5
Effect of increasing bacterial cell count and membrane area on MB removal % after the 48 h incubation period.
Figure 6
Figure 6
SEM images of bacteria-immobilized CA/PEO nanofibers after dye removal and fiber diameter distribution; (A) at low magnification, (B) at high magnification and (C) fiber diameter distribution.
Figure 7
Figure 7
Fitting of Langmuir model to the adsorption results of MB by CA/PEO nanofibers.
Figure 8
Figure 8
Reusability test results of the four usages on MB removal %.
Figure 9
Figure 9
Mechanism of methylene blue biodegradation by bacteria.
See this image and copyright information in PMC

References

    1. Ahmed Basha C, Bhadrinarayana NS, Anantharaman N, Meera Sheriffa Begum KM. Heavy metal removal from copper smelting effluent using electrochemical cylindrical flow reactor. J. Hazard. Mater. 2008;152:71–78. doi: 10.1016/j.jhazmat.2007.06.069. - DOI - PubMed
    1. Singh R, Singh R, Singh P. Bacterial decolorization of textile azo dye acid orange by staphylococcus hominis RMLRT03. Toxicol. Int. 2014;21:160. doi: 10.4103/0971-6580.139797. - DOI - PMC - PubMed
    1. Saratale RG, Saratale GD, Chang JS, Govindwar SP. Bacterial decolorization and degradation of azo dyes: A review. J. Taiwan Inst. Chem. Eng. 2011;42:138–157. doi: 10.1016/j.jtice.2010.06.006. - DOI
    1. Pirkarami A, Olya ME. Removal of dye from industrial wastewater with an emphasis on improving economic efficiency and degradation mechanism. J. Saudi Chem. Soc. 2017;21:S179–S186. doi: 10.1016/j.jscs.2013.12.008. - DOI
    1. Mishra A, Bajpai M. The flocculation performance of Tamarindus mucilage in relation to removal of vat and direct dyes. Bioresour. Technol. 2006;97:1055–1059. doi: 10.1016/j.biortech.2005.04.049. - DOI - PubMed