Dual time point [18F]FLT-PET for differentiating proliferating tissues vs non-proliferating tissues

Abstract

Purpose: For differentiating tumor from inflammation and normal tissues, fluorodeoxyglucose ([¹⁸F]FDG) dual time point PET could be helpful. Albeit [¹⁸F]FLT is more specific for tumors than [¹⁸F]FDG; we explored the role of dual time point [¹⁸F]FLT-PET for discriminating benign from malignant tissues.

Methods: Before any treatment, 85 womens with de novo unifocal breast cancer underwent three PET acquisitions at 33.94 ± 8.01 min (PET30), 61.45 ± 8.30 min (PET60), and 81.06 ± 12.12 min (PET80) after [¹⁸F]FLT injection. Semiquantitative analyses of [¹⁸F]FLT uptake (SUV) were carried out on tumors, liver, bone marrow (4th thoracic vertebra (T4) and humeral head), descending thoracic aorta, muscle (deltoid), and contralateral normal breast. Repeated measures ANOVA tests and Tukey's posttests were used to compare SUVmax of each site at the three time points.

Results: There was a significant increase in SUVmax over time for breast lesions (5.58 ± 3.80; 5.97 ± 4.56; 6.19 ± 4.42; p < 0.0001) (m ± SD for PET30, PET60, and PET80, respectively), and bone marrow (for T4, 8.21 ± 3.17, 9.64 ± 3.66, 10.85 ± 3.63, p < 0.0001; for humeral head, 3.36 ± 1.79, 3.87 ± 1.89, 4.39 ± 2.00, p < 0.0001). A significant decrease in SUVmax over time was observed for liver (6.79 ± 2.03; 6.24 ± 1.99; 5.57 ± 1.74; p < 0.0001), muscle (0.95 ± 0.28; 0.93 ± 0.29; 0.86 ± 0.20; p < 0.027), and aorta (1.18 ± 0.34; 1.01 ± 0.32; 0.97 ± 0.30; p < 0.0001). No significant difference was observed for SUVmax in contralateral breast (0.8364 ± 0.40; 0.78 ± 0.38; 0.80 ± 0.35).

Conclusion: [¹⁸F]FLT-SUVmax increased between 30 and 80 min only in proliferating tissues. This could be helpful for discriminating between residual tumor and scar tissue.

Keywords: Breast cancer; FLT; Fluorothymidine; Positron emission tomography.

Fig. 1

Primary breast tumor SUVmax differences between PET80 and PET30 (∆SUVmax). ∆SUVmax is plotted vs PET30 SUVmax. Note that 24 patients showed a decrease in FLT uptake, even though these decreases are marginal or of low amplitude. However, 50% of the patients correspond to an increase in FLT uptake between 30 and 60 min (79% are ductal carcinoma, and 58% are SBR II)