Paenibacillus oralis sp. nov., Isolated from Human Subgingival Dental Plaque of Gingivitis Lesion

Abstract

A Gram-stain-negative, facultative anaerobic, spore-forming, motile, and rod-shaped bacterium, strain ChDC PVNT-B20^T, was isolated from the human subgingival dental plaque of a gingivitis lesion. Phylogenetic analysis based on the 16S ribosomal RNA gene (16S rDNA) showed that the strain belonged to the genus Paenibacillus. BLAST analysis of 16S rDNA sequence of the strain displayed high identity to those of Paenibacillus faecis DSM 23593^T (97.7% similarity) and Paenibacillus macerans ATCC 8244^T (97.6% similarity). Draft genome of strain ChDC PVNT-B20^T was composed of 8,112,407 bp. The DNA G+C content of the strain was 51.3 mol%. Average nucleotide identity values between strain ChDC PVNT-B20^T and P. faecis DSM 23593^T or P. macerans ATCC 8244^T were 75.71% and 91.5%, respectively. Genome-to-genome distance values between strain ChDC PVNT-B20^T and P. faecis DSM 23593^T or P. macerans ATCC 8244^T were 21.6% (19.3-24.0%) and 44.9% (42.3-47.4%), respectively. Major cellular fatty acids of strain ChDC PVNT-B20^T were anteiso-C_15:0 (43.4%), C_16:0 (16.6%), iso-C_16:0 (14.4%), and anteiso-C_17:0 (12.4%). The sole respiratory quinone of the strain was menaqinone-7. Major polar lipids of the strain were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), and one unidentified glycolipid (GL). Minor polar lipids were one unidentified aminolipid (AL), one unidentified phospholipid (PL), and three unidentified lipids (L1-L3). Based on these results, strain ChDC PVNT-B20^T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus oralis sp. nov. is proposed. Type strain is ChDC PVNT-B20^T (= KCOM 3021^T = JCM 33462 ^T).