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. 2019 Dec 11;9(12):1122.
doi: 10.3390/ani9121122.

Effects of Dietary Selenium Deficiency or Excess on Selenoprotein Gene Expression in the Spleen Tissue of Pigs

Zhuang Lu  1 , Pengzu Wang  1 , Teng Teng  1 , Baoming Shi  1 , Anshan Shan  1 , Xin Gen Lei  2
Affiliations

Effects of Dietary Selenium Deficiency or Excess on Selenoprotein Gene Expression in the Spleen Tissue of Pigs

Zhuang Lu et al. Animals (Basel). .

Abstract

To evaluate the effects of dietary Se deficiency and excess on the mRNA levels of selenoproteins in pig spleen tissues, 20 healthy uncastrated boars (Duroc × Landrace × Yorkshire, 10 ± 0.72 kg) were randomly divided into four groups (5 pigs per group). The pigs were fed a Se deficient corn-soybean basal feed (Se content <0.03 mg/kg) or basal feed with added sodium selenite at 0.3, 1.0, or 3.0 mg Se/kg diet, respectively. The experiment lasted 16 weeks. The spleen tissue was collected to examine the mRNA expression levels of 24 selenoprotein genes at the end of the study. Compared with pigs in other groups, those fed with the 1.0 mg Se/kg diet had higher mRNA levels of glutathione peroxidase 1 (Gpx1), glutathione peroxidase 2 (Gpx2), deiodinase type II (Dio2), thioredoxin reductase 3 (Txnrd3), selenoprotein H (Selh), selenoprotein N, 1 (Sepn1), selenoprotein P1 (Sepp1), and selenoprotein V (Selv) in the spleen (p < 0.05). Dietary Se deficiency resulted in lower mRNA levels of Gpx1, Gpx2, glutathione peroxidase 3 (Gpx3), Dio2, thioredoxin reductase 2 (Txnrd2), Txnrd3, Selh, selenoprotein I (Seli), selenoprotein K (Selk), selenoprotein M (Selm), Sepn1, Sepp1, and Selv in the spleen than the other three groups. Dietary Se levels did not affect the mRNA levels of glutathione peroxidase 4 (Gpx4), deiodinase type I (Dio1), deiodinase type III (Dio3), selenophosphate synthetase 2 (Sephs2), thioredoxin reductase 1 (Txnrd1), selenoprotein O (Selo), selenoprotein S (Sels), selenoprotein W (Selw), selenoprotein X (Selx), and selenoprotein 15 (Sel15) in the spleen (p > 0.05). Dietary Se levels can affect the transcription levels of 14 selenoprotein genes in the spleen of pigs.

Keywords: pig; selenium; selenoprotein; spleen.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Effects of dietary Se concentrations on mRNA levels of selenoprotein genes in the spleen of pigs at wk 16. Data are means ± SDs, n = 5. Means stands for a gene without a common letter differ, p < 0.05. Gpx: glutathione peroxidase; Dio: deiodinase type; Sephs: Selenophosphate synthetase; Txnrd: Thioredoxin reductase; Selh: Selenoprotein H; Seli: Selenoprotein I; Selk: Selenoprotein K; Selm: Selenoprotein M; Sepn1: Selenoprotein N, 1; Selo: Selenoprotein O; Sepp1: Selenoprotein P1; Sels: Selenoprotein S; Selt: Selenoprotein T; Selv: Selenoprotein V; Selw: Selenoprotein W; Selx: Selenoprotein X; Sep15: Selenoprotein 15.
Figure 1
Figure 1
Effects of dietary Se concentrations on mRNA levels of selenoprotein genes in the spleen of pigs at wk 16. Data are means ± SDs, n = 5. Means stands for a gene without a common letter differ, p < 0.05. Gpx: glutathione peroxidase; Dio: deiodinase type; Sephs: Selenophosphate synthetase; Txnrd: Thioredoxin reductase; Selh: Selenoprotein H; Seli: Selenoprotein I; Selk: Selenoprotein K; Selm: Selenoprotein M; Sepn1: Selenoprotein N, 1; Selo: Selenoprotein O; Sepp1: Selenoprotein P1; Sels: Selenoprotein S; Selt: Selenoprotein T; Selv: Selenoprotein V; Selw: Selenoprotein W; Selx: Selenoprotein X; Sep15: Selenoprotein 15.
Figure 1
Figure 1
Effects of dietary Se concentrations on mRNA levels of selenoprotein genes in the spleen of pigs at wk 16. Data are means ± SDs, n = 5. Means stands for a gene without a common letter differ, p < 0.05. Gpx: glutathione peroxidase; Dio: deiodinase type; Sephs: Selenophosphate synthetase; Txnrd: Thioredoxin reductase; Selh: Selenoprotein H; Seli: Selenoprotein I; Selk: Selenoprotein K; Selm: Selenoprotein M; Sepn1: Selenoprotein N, 1; Selo: Selenoprotein O; Sepp1: Selenoprotein P1; Sels: Selenoprotein S; Selt: Selenoprotein T; Selv: Selenoprotein V; Selw: Selenoprotein W; Selx: Selenoprotein X; Sep15: Selenoprotein 15.
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