In vitro assessment of oral and respiratory bioaccesibility of trace elements of environmental concern in Greek fly ashes: Assessing health risk via ingestion and inhalation

Abstract

Fly ash engender significant environmental and human health problems due to enhanced contents of potentially harmful trace elements (TrElems). This study aims to evaluate human exposure to TrElems via a combined ingestion (i.e., oral bioaccessibility) and inhalation (i.e., respiratory bioaccessibility) pathway. Five fly ash samples were collected from power plants operating in the main lignite basins of Greece, while the ingestible (<250 μm) and inhalable (<10 μm) particle size fractions were utilized. The Unified Bioaccessibility Method (UBM) was utilized to assess the oral bioaccessibility, while the respiratory bioaccessible fractions were extracted using the Artificial Lysosomal Fluid (ALF). All studied FAs exhibited significantly higher contents in Ba, Cr, Ni, V and Zn. Cadmium was presented relative enriched in the finer size fraction (<10 μm), while Ba, Co, Cr, Cu, Mn, Ni and V were depleted. The UBM-extractable concentrations fluctuated greatly among the studied FAs, while notably lower bioaccessible contents were recorded in the gastrointestinal phase. On the other hand, ALF-extractable concentrations were surprisingly higher than the corresponding UBM-extractable ones in the gastric phase. The oral bioaccessibility of the studied TrElems ranged from 12.5 to 100%, while respiratory bioaccessibility presented high values exceeding 45% on average. A significant effect of fly ash type on human bioaccessibility was revealed. Thus, high-Ca FAs exhibited significantly higher bioaccessibility of the studied TrElems via ingestion, while a relatively higher bioaccessibility via inhalation was observed for high-Si FAs. Regarding non-carcinogenic health risk via ingestion and inhalation, Cr and Co exhibited the highest HQ_ing and HQ_inh values, however there were significantly lower than safe level (HQ < 1). On the contrary, Cr was the dominant contributor to carcinogenic risk with CR values being well above threshold or even tolerable risk levels.

Keywords: Cancer risk; Fly ash; Health risk; Human bioaccessibility; Trace element.