Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Dec 5:61:1-26.
doi: 10.3897/mycokeys.61.46446. eCollection 2019.

Descriptions of five new species in Entoloma subgenus Claudopus from China, with molecular phylogeny of Entoloma s.l

Xiao-Lan He  1 , Egon Horak  2 , Di Wang  1 , Tai-Hui Li  3 , Wei-Hong Peng  1 , Bing-Cheng Gan  1
Affiliations

Descriptions of five new species in Entoloma subgenus Claudopus from China, with molecular phylogeny of Entoloma s.l

Xiao-Lan He et al. MycoKeys. .

Erratum in

Abstract

Entoloma subgenus Claudopus is widely distributed, yet the taxonomy and systematics of its species are still poorly documented. In the present study, more than forty collections of Claudopus were gathered in China and subsequently analysed, based on morphological and molecular data. The results revealed first a high level of species diversity of Claudopus in China and second, there is a wide ecological range regarding the substrates and the habitats ranging from temperate, tropical to subalpine locations. Based on morphological and molecular evidence, five novel species from China are proposed, viz. E. conchatum, E. flabellatum, E. gregarium, E. pleurotoides and E. reductum. Molecular phylogeny of Entoloma s.l. was also reconstructed, based on 187 representatives of Entoloma s.l. by employing the combined ITS, LSU, mtSSU and RPB2 sequences. Ten monophyletic clades (Claudopus, Leptonia, Nolanea, Cuboid-spored Inocephalus, "Alboleptonia", Cyanula, Pouzarella, Rhodopolia, Prunuloides and Rusticoides) were recovered, while 13 taxa could not be placed in any defined clades. The results confirmed that Claudopus in a traditional morphological sense is not monophyletic and the Rusticoides-group, previously considered within Claudopus, formed a separate clade; but section Claudopus and relatives of E. undatum belong to a distinctive monophyletic group. Despite some monophyletic groups in Entoloma s.l. being distinctive in both morphology and molecular phylogeny, they were still treated as subgenera of Entoloma s.l. temporarily, because accepting them as genera will make Entoloma s.l. paraphyletic.

Keywords: Entolomataceae; ecology; multi-gene analyses; systematics; taxonomy.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Basidiomes of Claudopus species a Basidiomes of E. conchatum on soil (SAAS 1712) b Basidiomes of E. conchatum on stem of live Pinus (SAAS 1014) c Pileus of E. flabellatum (SAAS 1501) d Lamellae of E. flabellatum (SAAS 1080) e Basidiomes of C. gregarious on bark-wood of live Castanopsis (SAAS 1220) f Red droplets on the lamellar edges of E. gregarium (SAAS 1493) g Basidiomes of E. pleurotoides on decaying bark-wood of Castanopsis (SAAS 1215) h Basidiomes of E. pleurotoides on bark-wood of live Castanopsis (SAAS 1252) i Basidiomes of E. reductum on decaying stump of Castanopsis (holotype, SAAS 1091) j Mature basidiomes of E. reductum on rock (SAAS 2068) k Young basidiomes of E. reductum on soil (SAAS 1016) l Lamellae of E. byssisedum var. microsporum (SAAS 1828) m Basidiomes of E. byssisedum var. microsporum on decaying stump of Betula (SAAS 1160).
Figure 2.
Figure 2.
Microscopic structures of Entoloma conchatum (holotype) a Basidiospores b Basidia c Pileipellis.
Figure 3.
Figure 3.
Microscopic structures of Entoloma flabellatum (holotype) a Basidiospores b Basidia c Pileipellis.
Figure 4.
Figure 4.
Microscopic structures of Entoloma gregarium (holotype) a Basidiospores b Basidia c Pileipellis.
Figure 5.
Figure 5.
Microscopic structures of Entoloma pleurotoides (holotype): a Basidia b Basidiospores c Pileipellis.
Figure 6.
Figure 6.
Microscopic structures of Entoloma reductum (holotype) a Basidiospores b Pileipellis.
Figure 7.
Figure 7.
Microscopic structures of Entoloma byssisedum var. microsporum (SAAS 1279) a Basidiospores b Basidia c Pileipellis.
Figure 8.
Figure 8.
Phylogenetic reconstruction of Claudopus based on ITS sequences. Maximum parsimony bootstrap values (BS > 50%) are indicated above or below the branches, new species are in bold.
Figure 9.
Figure 9.
Cladogram based on the combined ITS, LSU, RPB2 and mtSSU sequences resulting from ML analysis. New species of Entoloma subgenus Claudopus are in bold. MPBS support values (> 50%), RAxML BS support values (> 50%) and Bayesian posterior probability values (BPP> 0.90) are indicated above or below branches as MPBS/RAxML BS/BPP.
See this image and copyright information in PMC

References

    1. Baroni TJ, Matheny PB. (2011) A re-evaluation of gasteroid and cyphelloid species of Entolomataceae from eastern North America. Harvard Papers in Botany 16: 293–310. 10.3100/0.25.016.0205 - DOI
    1. Baroni TJ, Hofstetter V, Largent DL, Vilgalys R. (2011) Entocybe is proposed as a new genus in the Entolomataceae (Agaricomycetes, Basidiomycota) based on morphological and molecular evidence. North Am Fungi 6(12): 1–19. 10.2509/naf2011.006.012 - DOI
    1. Chen JJ, Cui BK, Dai YC. (2016) Global diversity and molecular systematics of Wrightoporia s. l. (Russulales, Basidiomycota). Persoonia 37: 21–36. 10.3767/003158516X689666 - DOI - PMC - PubMed
    1. Co-David C, Langeveld D, Noordeloos ME. (2009) Molecular phylogeny and spore evolution of Entolomataceae. Persoonia 23: 147–176. 10.3767/003158509X480944 - DOI - PMC - PubMed
    1. Darriba D, Taboada GL, Doallo R, Posada D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9: 772. 10.1038/nmeth.2109 - DOI - PMC - PubMed

LinkOut - more resources