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. 2019 Oct;48(10):1879-1884.

Evaluation of (GTG) 5-PCR for Genotyping of Klebsiella pneumonia Strains Isolated from Patients with Urinary Tract Infections

Reza Ranjbar  1 , Davoud Afshar  2
Affiliations

Evaluation of (GTG) 5-PCR for Genotyping of Klebsiella pneumonia Strains Isolated from Patients with Urinary Tract Infections

Reza Ranjbar et al. Iran J Public Health. 2019 Oct.

Abstract

Background: Klebsiella pneumonia is one of the common causes of pneumonia and bacteremia in intensive care patients. The present study was aimed to determine the capability of (GTG) 5-PCR assay for molecular typing of K. pneumonia strains isolated from patients with urinary tract infections.

Methods: In this descriptive-sectional study, K. pneumoniae strains were collected from hospitalized patients with urinary tract infection in Baqiyatallah Hospital, Tehran, Iran during 2017-2018. Isolates were identified by conventional microbiological tests. Bacterial DNA was extracted using boiling method and (GTG) 5-PCR assay was used for subtyping of the isolates. For clustering of isolates, dendrogram was generated according to the un-weighted pair group method with arithmetic (UPGMA).

Results: Overall, 88 K. pneumoniae isolates were isolated and subjected to the molecular typing study. The (GTG) 5-PCR assay was able to differentiate the K. pneumoniae strains into 9 clusters including G1-G9. Genotype clusters G4 and G9 consist of highest (26) and lowest (1) number isolate, respectively.

Conclusion: The K. pneumonia strains isolated under the study belonged to various clones and the (GTG) 5-PCR assay as simple and rapid method can be a powerful tool for molecular typing of K. pneumoniae strains.

Keywords: (GTG) 5-PCR assay; Klebsiella pneumoniae; Molecular typing.

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Conflict of interest statement

Conflict of interest The authors declare that there are no conflicts of interest.

Figures

Fig. 1:
Fig. 1:
The (GTG) PCR fingerprints of several K. pneumoniae isolates (20–26)
Fig. 2:
Fig. 2:
Dendrogram based on (GTG)5-PCR fingerprints derived from 19 K. pneumoniae clinical isolates. Comparison method: Dice Clustering method: UPGMA
Fig. 2:
Fig. 2:
Dendrogram based on (GTG)5-PCR fingerprints derived from 19 K. pneumoniae clinical isolates. Comparison method: Dice Clustering method: UPGMA
See this image and copyright information in PMC

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