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. 2020 May;104(5):409-419.
doi: 10.1111/ejh.13371. Epub 2020 Feb 22.

Circulating extracellular vesicle-associated CD163 and CD206 in multiple myeloma

Sarah L Kvorning  1   2 , Marlene C Nielsen  1 , Niels F Andersen  3 , Marianne Hokland  2 , Morten N Andersen  1   2   3 , Holger J Møller  1
Affiliations

Circulating extracellular vesicle-associated CD163 and CD206 in multiple myeloma

Sarah L Kvorning et al. Eur J Haematol. 2020 May.

Abstract

Objectives: Extracellular vesicles (EVs) are important for intercellular signalling in cancer. Tumour-associated macrophages, expressing the haemoglobin-haptoglobin and mannose receptors CD163 and CD206, are crucial for cancer progression. We recently identified CD163 on EVs in the circulation as a fraction of total soluble CD163 (sCD163). Here, we investigated the presence of CD163 and CD206-positive EVs (EV-CD163, EV-CD206) in patients with multiple myeloma (MM).

Methods: We enrolled patients with MM (n = 32), monoclonal gammopathy of undetermined significance (MGUS) (n = 8) and healthy donors (n = 16). Plasma protein levels were determined by ELISA before and after vesicle precipitation. Monocytes were examined by flow cytometry, and leucocyte CD163 mRNA by qPCR.

Results: Fractions of EV-CD163 and EV-CD206 were significantly elevated in patients with newly diagnosed MM (median = 39.8%, 76.5%, respectively) compared to patients with relapse (15.6%, P = .02, 42.5%, P = .003), remission (16.9%, P < .0001, 25.2%, P < .0001), MGUS (17.8%, P < .01, 33.1%, P = .0005) and healthy donors (14.8%, P < .0001, 35.5%, P < .0001). Whole blood CD163 mRNA did not vary between the groups. The intermediate monocyte subset showed a higher CD163 expression in newly diagnosed patients.

Conclusions: Our results indicate that macrophage-derived EVs may play a role in the late phase of malignant progression of MM, and encourage further EV investigations in functional experiments.

Keywords: CD163; CD206; extracellular vesicles; molecular cytogenetics; multiple myeloma; plasma cell neoplasms; tumour-associated macrophage.

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References

REFERENCES

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