Production of a recombinant antigen of Echinococcus multilocularis with high immunodiagnostic sensitivity and specificity

Abstract

A cDNA library derived from mRNA of metacestodes from Echinococcus multilocularis was constructed in the Escherichia coli expression vector lambda gt11 and screened with human patients' sera. The recombinant proteins of 11 positive phages were further evaluated for their potential as immunodiagnostic reagent. One isolated clone (from lysogen II/3) synthesized a fusion protein which was rapidly degraded. This intracellular degradation process provided two distinct polypeptides with Mr of about 31,000 and 33,000, both showing strong binding activity with specific patients' antibodies. The diagnostic value of the II/3-antigen was evaluated by mini-Western-blot with sera from 41 patients infected with E. multilocularis and sera from 77 patients with other helminthic infections, resulting in a diagnostic sensitivity of 98% and an over-all specificity of 96%. These results suggest the usefulness of the recombinant II/3-antigen for immunodiagnosis of human alveolar echinococcosis.