Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Nov;31(2):127-31.
doi: 10.1016/0166-6851(88)90163-6.

Nucleoside analysis of DNA from Trypanosoma brucei and Trypanosoma equiperdum

M Crozatier  1 , R J De Brij, L Den Engelse, P J Johnson, P Borst
Affiliations

Nucleoside analysis of DNA from Trypanosoma brucei and Trypanosoma equiperdum

M Crozatier et al. Mol Biochem Parasitol. 1988 Nov.

Abstract

We have digested trypanosome DNA with a combination of pancreatic DNase I, nuclease P1 and bovine alkaline phosphatase and fractionated the resulting nucleosides on a Supelcosil LC-18-S column by high pressure liquid chromatography. We find less than 0.1% unusual nucleosides, both in Trypanosoma brucei and in a Trypanosoma equiperdum stock, in contrast to a previous report of an unusual nucleoside replacing dC at 1.3% of total nucleosides in T. equiperdum. Our results agree with previous suggestions that the modification of inactive telomeric expression sites for variant-specific surface glycoprotein genes in T. brucei only affects a very small fraction of the total DNA.

PubMed Disclaimer

Publication types