Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra

Munsoor A Hanifa^{1

2

3}, Raluca G Maltesen², Bodil S Rasmussen^{2

3}, Katrine B Buggeskov⁴, Hanne B Ravn⁴, Martin Skott⁵, Søren Nielsen⁶, Jørgen Frøkiær⁷, Troels Ring^{8

9}, Reinhard Wimmer¹⁰

Affiliations

¹ Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, 9220, Aalborg, Denmark.
² Department of Anesthesia and Intensive Care Medicine, Aalborg University Hospital, 9000, Aalborg, Denmark.
³ Department of Clinical Medicine, Aalborg University, 9000, Aalborg, Denmark.
⁴ Department of Cardiothoracic Anesthesiology, Rigshospitalet, Copenhagen University Hospital, 2100, Copenhagen, Denmark.
⁵ Department of Urology, Aarhus University Hospital, 8250, Aarhus N, Denmark.
⁶ Meta-IQ, Fredensgade 9, 8000, Aarhus C, Denmark.
⁷ Department of Clinical Medicine, Aarhus University, 8200, Aarhus N, Denmark.
⁸ Department of Biomedicine, Aarhus University, 8000, Aarhus C, Denmark.
⁹ The Center for Critical Care Nephrology, Department of Critical Care Medicine, University of Pittsburgh, Pittsburg, PA, 15261, USA.
¹⁰ Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, 9220, Aalborg, Denmark. rw@bio.aau.dk.

PMID: 31858270
DOI: 10.1007/s11306-019-1629-8

Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra

Munsoor A Hanifa et al. Metabolomics. 2019.

. 2019 Dec 19;16(1):7.

doi: 10.1007/s11306-019-1629-8.

Authors

Affiliations

¹ Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, 9220, Aalborg, Denmark.
² Department of Anesthesia and Intensive Care Medicine, Aalborg University Hospital, 9000, Aalborg, Denmark.
³ Department of Clinical Medicine, Aalborg University, 9000, Aalborg, Denmark.
⁴ Department of Cardiothoracic Anesthesiology, Rigshospitalet, Copenhagen University Hospital, 2100, Copenhagen, Denmark.
⁵ Department of Urology, Aarhus University Hospital, 8250, Aarhus N, Denmark.
⁶ Meta-IQ, Fredensgade 9, 8000, Aarhus C, Denmark.
⁷ Department of Clinical Medicine, Aarhus University, 8200, Aarhus N, Denmark.
⁸ Department of Biomedicine, Aarhus University, 8000, Aarhus C, Denmark.
⁹ The Center for Critical Care Nephrology, Department of Critical Care Medicine, University of Pittsburgh, Pittsburg, PA, 15261, USA.
¹⁰ Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, 9220, Aalborg, Denmark. rw@bio.aau.dk.

PMID: 31858270
DOI: 10.1007/s11306-019-1629-8

Abstract

Background: In our metabolomics studies we have noticed that repeated NMR acquisition on the same sample can result in altered metabolite signal intensities.

Aims: To investigate the reproducibility of repeated NMR acquisition on selected metabolites in serum and plasma from two large human metabolomics studies.

Methods: Two peak regions for each metabolite were integrated and changes occurring after reacquisition were correlated.

Results: Integral changes were generally small, but serum citrate signals decreased significantly in some samples.

Conclusions: Several metabolite integrals were not reproducible in some of the repeated spectra. Following established protocols, randomising analysis order and biomarker validation are important.

Keywords: Metabolomics; NMR; Plasma; Reproducibility; Serum.

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References

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Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra

Affiliations

Citrate NMR peak irreproducibility in blood samples after reacquisition of spectra

Authors

Affiliations

Abstract

References

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