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Comparative Study
. 2019 Dec;98(51):e17944.
doi: 10.1097/MD.0000000000017944.

The expression of mouse double minute 2 homolog and P73 had no correlation with growth arrest DNA damage-inducible gene 45α in patients with non-small-cell lung carcinoma: A STROBE-compliant study

Bo Wang  1 , Chaoyang Liang  2 , Huifeng Liu  3 , Jixing Lin  2 , Bailin Wang  2 , Kaijie Fan  1 , Zhipeng Ren  1 , Bin Wang  1 , Tong Li  1 , Kang Qi  1 , Xiaodong Tian  2
Affiliations
Comparative Study

The expression of mouse double minute 2 homolog and P73 had no correlation with growth arrest DNA damage-inducible gene 45α in patients with non-small-cell lung carcinoma: A STROBE-compliant study

Bo Wang et al. Medicine (Baltimore). 2019 Dec.

Abstract

To investigate the difference in messenger ribonucleic acid (mRNA) and protein expression of growth arrest DNA damage-inducible gene 45α (GADD45α), mouse double minute 2 homolog (MDM2), and P73 in cancer and cancer-adjacent tissues in patients with non-small-cell lung carcinoma (NSCLC).We compared the mRNA expression of GADD45α and MDM2 and the protein expression of GADD45α, MDM2, and P73 in lung cancer and cancer-adjacent tissues in NSCLC patients by quantitative real-time PCR, immunohistochemistry (IHC), and Western Blot (WB). We analyzed GADD45α, MDM2, and P73 expression in patients with different pathological types of NSCLC, and the correlation of these genes with gender, smoking history, and TNM/T stages.IHC results suggested that MDM2 protein expression significantly increased in cancer tissues in female patients (P = .01), but not in male patients. In addition, WB results indicated that P73 protein expression significantly decreased in cancer tissues in patients with adenocarcinoma (P = .03), but not squamous carcinoma.MDM2 and P73 protein levels were differentially regulated in cancer and cancer-adjacient tissues in patients with sub types of NSCLC. There was no significant difference in GADD45α expression between cancer and cancer-adjacent tissues in NSCLC patients.

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Conflict of interest statement

The authors have no conflicts of interest to disclose.

Figures

Figure 1
Figure 1
The images of GADD45α, MDM2, and P73 expression between cancer and cancer-adjacent tissues measured by IHC. Blue dot indicated nucleus, yellow area indicated the region of interest. GADD45α = growth arrest DNA damage-inducible gene 45α; IHCα = immunohistochemistry, MDM2α = mouse double minute 2 homolog.
Figure 2
Figure 2
GADD45α, MDM2, and P73 protein expression measured by IHC in all patients. (A) The IOD/total area value of MDM2, GADD45α, and P73 between cancer and cancer-adjacent tissues in all patients analyzed by IPP software; (B) the IHC images of MDM2 between cancer and cancer-adjacent tissues in female patients; (C) the IOD/total area value of MDM2 between cancer and cancer-adjacent tissues in female patients; ∗P < .05, indicating a significant difference. GADD45αα = growth arrest DNA damage-inducible gene 45α, IHCα = immunohistochemistry, IODα = integrated optical density, IPPα = image-pro plus, MDM2α = mouse double minute 2 homolog.
Figure 3
Figure 3
WB results and quantitative analysis. (A) Image of Western blot showed the protein expression of GADD45α, MDM2, and P73. Cancer and cancer-adjacent tissue samples were from 8 patients. (B) The quantitative analysis (Gray value) of MDM2, GADD45α, and P73 in all patients; (C) the quantitative analysis (Gray value) of P73 between cancer and cancer-adjacent tissues in patients with SCC and ACC. T: tumor tissues; N: nontumor tissues. ∗P < .05, indicating a significant difference. ACCα = adenocarcinoma, GADD45αα = growth arrest DNA damage-inducible gene 45α; MDM2α = mouse double minute 2 homolog, SCCα = squamous carcinoma.
See this image and copyright information in PMC

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