CD247 expression is associated with differentiation and classification in ovarian cancer

Abstract

Ovarian cancer (OC) is one of the most common malignant tumors in female reproductive system and most OC cases are diagnosed at an advanced stage with the overall 5-year survival rate below 40%. The function of CD247 enhances T-cell antigen receptor (TCR) signaling cascade and it is necessary for assembling of the TCR/CD3 complex on the surface of T lymphocytes. It is well established that defective CD247 function leads to impaired activation of T cells upon engagement of the TCR.Flow cytometry was used to examine the difference of CD247 T lymphocyte between the OC and ovarian cyst, immunohistochemistry analysis was used to investigate the correlation between CD247 expression and clinicopathologic features of epithelial OC patients.Our study showed that the expression of CD247 in peripheral blood lymphocytes from patients with OC is decreased compared with ovarian cyst patients and the expression of CD247 in tumor infiltrating lymphocytes with cancer tissue is decreased compared with adjacent tissues. We showed that abnormal expression of CD247 was related with differentiation and classification in OC.Our findings suggested that CD247-targeted treatment could be used as a potential therapeutic strategy for OC.

Figure 1

CD3⁺CD45⁺, CD4⁺CD247⁺, and CD8⁺CD247⁺T-cell detection with flow cytometry is shown. T = peripheral blood mononuclear cell (PBMC) from the ovarian cancer patients, N = PBMC from the ovarian cyst patients. Results are presented as mean ± standard deviation. ^∗P < .05 (Student t test).

Figure 2

CD3⁺CD45⁺, CD3⁺CD247⁺, CD4⁺CD247⁺, and CD8⁺CD247⁺T-cell detection with flow cytometry is shown. C = Tumor-infiltrating lymphocyte (TIL) from ovarian cancer tissues, A = TIL from adjacent tissues, P = T cells from peripheral blood mononuclear cell with ovarian cancer. Results are presented as mean ± standard deviation. ^∗P < .05, ^∗∗P < .01 (Student t test).

Figure 3

CD247 immunohistochemical staining in different types of ovarian cancer. (A) Serous adenocarcinoma, (B) mucinous adenocarcinoma, (C) endometrioid adenocarcinoma, and (D) clear cell carcinoma.

Figure 4

CD247 immunohistochemical staining in 3 types differentiation of ovarian cancer. (A) Poorly differentiated case. (B) Moderately differentiated case. (C) Well-differentiated case.