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. 2019 Dec 18;21(1):17.
doi: 10.3390/ijms21010017.

Association of Complement Factor D and H Polymorphisms with Recurrent Pregnancy Loss

Hee Young Cho  1 , Han Sung Park  2 , Eun Ju Ko  2 , Chang Soo Ryu  2 , Jung Oh Kim  2 , Young Ran Kim  1 , Eun Hee Ahn  1 , Woo Sik Lee  3 , Nam Keun Kim  2
Affiliations

Association of Complement Factor D and H Polymorphisms with Recurrent Pregnancy Loss

Hee Young Cho et al. Int J Mol Sci. .

Abstract

Recurrent pregnancy loss (RPL) is defined as two or more consecutive pregnancy losses prior to 20 weeks of gestation, and the incidence of RPL is estimated at 1% of all pregnancies. While the etiologies of RPL are diverse, immune function is considered to be an important cause of RPL. In particular, the complement system is essential for stable development of the placenta and fetus. Moreover, complement factor D (CFD) and complement factor H (CFH) are important regulators of the complement system and are associated with diseases, such as age-related macular degeneration. Therefore, we investigated whether polymorphisms of CFD and CFH are associated with RPL in 412 women with RPL and 384 control women. Genotyping of three polymorphisms (CFD rs2230216, CFH rs1065489, and CFH rs1061170) was performed by TaqMan probe real-time PCR and PCR-restriction fragment length polymorphism. Association of three polymorphisms with RPL was evaluated by statistical analysis. The GT/TC genotype combination of CFH rs1065489 G>T/CFH rs1061170 T>C was associated with a decreased risk of RPL occurrence compared with reference genotypes (adjusted odds ratio [AOR] = 0.439; 95% confidence interval [CI] = 0.238-0.810; p = 0.008), and this association remained significant after adjustment for multiple comparisons using false discovery rate (FDR) correction (p = 0.040). In addition, the CFH rs1065489G>T polymorphism is associated with homocysteine and prolactin level and CFH rs1061170 TC genotype is related to uric acid and triglycerides level in RPL patients. Therefore, those factors could be possible clinical risk factors in RPL patients.

Keywords: complement factor D; complement factor H; polymorphism; recurrent pregnancy loss.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Association between (a) homocysteine and (b) prolactin levels and the CFH rs1065489G>T polymorphisms in patients with RPL. * p < 0.05.
Figure 2
Figure 2
Association between (a) uric acid and (b) triglyceride levels and the CFH rs1061170T>C polymorphisms in patients with RPL. * p < 0.05.
See this image and copyright information in PMC

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