Correction to: Measles virus hemagglutinin triggers intracellular signaling in CD150-expressing dendritic cells and inhibits immune response

Abstract

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Fig. 2

CD150 modulates the Akt-signaling pathway in human DCs. DCs were cocultured with CHO/CHO-H cells a, c, d at a 1:5 ratio or stimulated with 10 μg/ml of anti-CD150 mAbs (clone IPO3) b at different times. Activation of signaling pathways via TLR2 and DC-SIGN receptors was blocked with specific anti-TLR2 c and anti-DC-SIGN d mAbs. FSL-1 was used as a specific ligand for TLR2 stimulation. Cell lysates were analyzed for pAkt (S473) expression by western blot with CD45 as a loading control. The level of pAkt was normalized against the level of CD45 using the TotalLab program e, f. e The results are expressed as the mean (±SD) from 3 to 6 independent experiments, and differences observed at 6, 12, and 24 h were statistically significant (*P < 0.05; **P < 0.001).

Fig. 4

CD150-mediated p38MAPK phosphorylation is decreased upon MV-H-CD150 interaction in DCs. DCs were either cocultured with CHO/CHO-H cells at a 1:5 ratio a, b or stimulated with 10 μg/ml of anti-CD150 IPO-3 antibody at different time points c. Activation of signaling pathways via the TLR2 receptor was blocked with specific anti-TLR2 antibodies d. Cell lysates were analyzed for pp38 MAPK (T180/Y182) expression by western blot with CD45 as a loading control. The level of ppMAPK38 was normalized against the level of CD45 using the TotalLab program e, f. e The results are expressed as the mean (±SD) from at least three independent experiments, and differences observed at 6 h were statistically significant (*P < 0.05, Student’s t test). g MP-1 cells were either stimulated with 10 μg/ml of anti-CD150 IPO-3 antibody or cocultured with CHO/CHO-H cells at a 1:5 ratio at different time points.