Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Mar:29:101625.
doi: 10.1016/j.pdpdt.2019.101625. Epub 2019 Dec 19.

Association of calcium hypochlorite, reciprocating instrumentation and photodynamic therapy: Antimicrobial analysis and effects on root dentin structure

Matheus Albino Souza  1 , Bruna Luiza Tozatti Lago  2 , Amália Pletsch  2 , Amanda Binotto  2 , Andressa Poletti  2 , Fernando Tolfo Rodrigues  2 , Rafaela Ricci  2 , Karolina Frick Bischoff  2 , Caroline Tumelero Dias  2 , Huriel Scartazzini Palhano  2 , Carlo Theodoro Raimundy Lago  2 , Ana Paula Farina  2 , Doglas Cecchin  2 , Juliane Bervian  2 , José Antônio Poli de Figueiredo  3
Affiliations

Association of calcium hypochlorite, reciprocating instrumentation and photodynamic therapy: Antimicrobial analysis and effects on root dentin structure

Matheus Albino Souza et al. Photodiagnosis Photodyn Ther. 2020 Mar.

Abstract

Introduction: This study aimed to evaluate the antimicrobial action of calcium hypochlorite(Ca[OCl]2) and sodium hypochlorite (NaOCl) with reciprocating instrumentation and photodynamic therapy(PDT), and its influence on root dentin structure.

Methods: One hundred and ten human teeth were used to antimicrobial evaluation, inoculated with Enterococcus faecalis and divided into 11 groups (n = 10): G1 - distilled water(control); G2-1 % NaOCl; G3-5.25 % NaOCl; G4-1 % Ca(OCl)2; G5-5.25 % Ca(OCl)2; G6-PDT; G7-distilled water + PDT; G8-1 % NaOCl + PDT; G9-5.25 % NaOCl + PDT; G10-1 % Ca(OCl)2+PDT; G11-5.25 % Ca(OCl)2+PDT. In all groups, except G6, the root canals were instrumented with #R50 Reciproc file in the working length in association with tested chemical auxiliary substance. The counting of colony forming units (CFUs) was performed to calculate the bacterial percentage reduction of each group. Following, 55 bovine teeth were used to microhardness evaluation and divided into the same 11 groups (n = 5), with no instrumentation and immersion in the tested protocols. The modification of root dentin microhardness was evaluated by using the Vicker tester. Finally, 33 bovine teeth were used to organic components evaluation and divided into the same 11 groups (n = 3), with no instrumentation and immersion in the tested protocols. The modification of organic components of root dentin was evaluated by light microscopy. Specific statistical analysis was performed for each evaluation.

Results: The higher bacterial reduction was observed in groups 3,4,5,8,9,10 and 11, with no statistical difference between them (p > 0.05). The higher microhardness reduction was observed in groups 8,9,10 e 11, with no statistical difference between them (p > 0.05). The highest modification on organic components was observed in groups 3,5,9 and 11, with no statistical difference between them (p > 0.05).

Conclusions: The association of Ca(OCl)2, reciprocating instrumentation and PDT promotes effective antimicrobial action. Moreover, lower modification was induced in microhardness and organic components of root dentin, by using Ca(OCl)2 in low concentration associated to PDT.

Keywords: Antimicrobial; Calcium hypochlorite; Microhardness; Organic; Photodynamic therapy; Reciprocating.

PubMed Disclaimer

Conflict of interest statement

Declaration of Competing Interest The authors declare no conflicts of interest

LinkOut - more resources