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. 1988 Nov;85(21):7852-6.
doi: 10.1073/pnas.85.21.7852.

Purification of an N-ethylmaleimide-sensitive protein catalyzing vesicular transport

M R Block  1 , B S Glick, C A Wilcox, F T Wieland, J E Rothman
Affiliations

Purification of an N-ethylmaleimide-sensitive protein catalyzing vesicular transport

M R Block et al. Proc Natl Acad Sci U S A. 1988 Nov.

Abstract

N-Ethylmaleimide (NEM) inhibits protein transport between successive compartments of the Golgi stack in a cell-free system. After inactivation of the Golgi membranes by NEM, transport can be rescued by adding back an appropriately prepared cytosol fraction. This complementation assay has allowed us to purify the NEM-sensitive factor, which we term NSF. The NEM-sensitive factor is a tetramer of 76-kDa subunits, and appears to act catalytically, one tetramer leading to the metabolism of numerous transport vesicles.

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References

    1. Science. 1975 Aug 1;189(4200):347-58 - PubMed
    1. Cell. 1988 Jul 15;54(2):221-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3870-4 - PubMed
    1. Science. 1981 Sep 11;213(4513):1212-9 - PubMed
    1. J Cell Biol. 1984 Jun;98(6):2142-7 - PubMed

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