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. 2020 Jan;38(1):35-38.
doi: 10.1038/s41587-019-0372-z. Epub 2019 Dec 23.

Highly multiplexed single-cell RNA-seq by DNA oligonucleotide tagging of cellular proteins

Jase Gehring  1   2 , Jong Hwee Park  2 , Sisi Chen  2 , Matthew Thomson  2 , Lior Pachter  3   4
Affiliations

Highly multiplexed single-cell RNA-seq by DNA oligonucleotide tagging of cellular proteins

Jase Gehring et al. Nat Biotechnol. 2020 Jan.

Abstract

We describe a universal sample multiplexing method for single-cell RNA sequencing in which fixed cells are chemically labeled by attaching identifying DNA oligonucleotides to cellular proteins. Analysis of a 96-plex perturbation experiment revealed changes in cell population structure and transcriptional states that cannot be discerned from bulk measurements, establishing an efficient method for surveying cell populations from large experiments or clinical samples with the depth and resolution of single-cell RNA sequencing.

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References

    1. Zheng, G. X. Y. et al. Massively parallel digital transcriptional profiling of single cells. Nat. Commun. 8, 14049 (2017). - DOI
    1. Svensson, V., Vento-Tormo, R. & Teichmann, S. A. Exponential scaling of single-cell RNA-seq in the past decade. Nat. Protoc. 13, 599–604 (2018). - DOI
    1. Han, X. et al. Mapping the Mouse Cell Atlas by Microwell-Seq. Cell 172, 1091–1107.e17 (2018). - DOI
    1. Cao, J et al. The single-cell transcriptional landscape of mammalian organogenesis. Nature 566, 496–502 (2019). - DOI
    1. Stoeckius, M. et al. Cell hashing with barcoded antibodies enables multiplexing and doublet detection for single cell genomics. Genome Biol. 19, 224 (2018). - DOI

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