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. 1977 Jan 20;474(2):165-72.
doi: 10.1016/0005-2787(77)90190-3.

Synthesis of guanosine 5'-diphosphate, 3'-diphosphate in spot mutants of Escherichia coli

H A de Boer, W J Weyer, J G de Boer, S van der Heide, M Gruber

Synthesis of guanosine 5'-diphosphate, 3'-diphosphate in spot mutants of Escherichia coli

H A de Boer et al. Biochim Biophys Acta. .

Abstract

The synthesis of ppGpp in spoT- mutants of Escherichia coli has been invesitgated. In these mutants the first-order rate constant for ppGpp breakdown is low, and pppGpp is barely detectable. It is shown that the rate of pppGpp, and hence ppGpp, synthesis is strongly reduced compared with that observed in spot+ strains. The low rate of magic spot synthesis satisfactorily explains the low levels of pppGpp in spoT- mutants. The pentaphosphate very probably is the precursor of ppGpp as it is in wild-type, i.e. spoT+, strains.

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