Targeted delivery and apoptosis induction of trans-resveratrol-ferulic acid loaded chitosan coated folic acid conjugate solid lipid nanoparticles in colon cancer cells

Abstract

The present study is aimed to study and to evaluate the colon cancer targeting efficacy of chitosan-coated-trans-resveratrol (RSV) and ferulic acid (FER) loaded SLNs (solid lipid nanoparticles) that conjugated with folic acid (FA) (C-RSV-FER-FA-SLNs) in suitable models (in vitro). The FA conjugation is performed using co-encapsulation method of stearic acid. Similarly, the prepared SLNs are exhibited better stability even under acidic conditions to exhibit their potentials to use as drug delivery system. Further, the optimized formulations (SLNs) are tested for physiochemical characterizations, which include FTIR, XRD, ¹HNMR, particle size, zeta potential, and drug release. In vitro anti-cancer studies using HT-29 cells including, fluorescence staining, flow cytometry, and western blot analysis revealed that the C-RSV-FER-FA-SLNs effectively involved and increased cytotoxicity in cancer cells that leads to induction of apoptosis as compared to free RSV-FER. Thus, it is reported that, the good stability under acidic conditions of this C-RSV-FER-FA-SLNs may serve as a promising candidate for novel nanodrug formulations in cancer therapy.

Keywords: Cellular apoptosis; Colon cancer; Cytotoxicity; Ferulic acid; Solid lipid nanoparticles (SLNs); Trans-resveratrol.