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. 2019 Oct-Dec;9(4):355-359.
doi: 10.1016/j.jobcr.2019.09.005. Epub 2019 Oct 4.

Mandible and iliac osteoblasts exhibit different Wnt signaling responses to LMHF vibration

Anute Pravitharangul  1 , Srisurang Suttapreyasri  2 , Chidchanok Leethanakul  3
Affiliations

Mandible and iliac osteoblasts exhibit different Wnt signaling responses to LMHF vibration

Anute Pravitharangul et al. J Oral Biol Craniofac Res. 2019 Oct-Dec.

Abstract

Objective: The jaw bones and long bones have distinct developmental origins and respond differently to mechanical stimuli. This study aimed to compare the Wnt signaling responses of human mandible osteoblasts and long bone osteoblasts to low-magnitude, high-frequency (LMHF) mechanical vibration in vitro.

Methods: Primary human osteoblast cultures were prepared from mandibular bone (n = 3) and iliac bone (n = 3) specimens (six individuals). Osteoblast cell lines were subjected to vibration (0, 30, 60, 90, or 120 Hz) for 30 min. After 24 h, cells were vibrated for 30 min again, then harvested immediately to quantify Wnt10b, Wnt5a and runt-related transcription factor 2 (RUNX2) mRNA expression, β-catenin protein expression and alkaline phosphatase (ALP) activity.

Results: Mandible and iliac osteoblasts responded differently to LMHF vibration: Wnt10b mRNA was upregulated by the frequency range tested; Wnt5a, β-catenin protein expression and RUNX2 mRNA expression were not altered. Furthermore, vibration upregulated ALP activity in mandible osteoblasts, but not in iliac osteoblasts.

Conclusions: This study demonstrates mandible osteoblasts and long bone osteoblasts respond differently to LMHF mechanical vibration in terms of Wnt signaling expression and ALP activity. Therefore, the effects of whole-body vibration on the long bones cannot be generalized to the jaw bones. Furthermore, osteoblast-like cells mediate the cellular responses to vibration, at least in part, by secreting extracellular signaling molecules.

Keywords: ALP, alkaline phosphatase; Alkaline phosphatase; Catenin; Human; LMHF vibration, low-magnitude, high-frequency vibration; Mandible; Osteoblast; RUNX2, runt-related transcription factor 2; Vibration; hIOBs, human iliac crest-derived mature osteoblast-like cells; hMOBs, human mandible-derived osteoblast-like cells.

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Conflict of interest statement

The authors have no potential conflicts of interest with respect to the authorship and/or publication of this article to declare.

Figures

Fig. 1
Fig. 1
Effect of LMHF vibration on the expression of canonical Wnt mRNAs in hMOBs and hIOBs. Cultured cells were exposed to 0, 30, 60, 90, or 120 Hz vibration for 30 min, cultured for 24 h, vibrated again for 30 min and then harvested. Real-time PCR analysis of A) Wnt10b and B) Wnt5a expression. Significant differences between different vibrational frequencies are indicated by *, ** and *** (p < 0.05, Kruskal-Wallis post-test). Significant differences between hMOBs and hIOBs are indicated by a and b (p < 0.05, Mann-Whitney U test).
Fig. 2
Fig. 2
Effect of LMHF mechanical vibration on A) β-catenin protein expression, B) runt-related transcription factor 2 (RUNX2) mRNA expression and C) alkaline phosphatase (ALP) activity in hMOBs and hIOBs. Cultured cells were exposed to 0, 30, 60, 90, or 120 Hz LMHF vibration for 30 min, cultured for 24 h, vibrated again for 30 min and then harvested. Significant differences between different vibrational frequency groups are indicated by * and ** (p < 0.05, Kruskal-Wallis post-test). Significant differences between hMOBs and hIOBs are indicated by a and b (p < 0.05, Mann-Whitney U test).
Fig. 3
Fig. 3
Effect of conditioned medium from vibrated cells on alkaline phosphatase (ALP) activity in hMOBs and hIOBs. Human MOBs and hIOBs were cultured for 24 h in the supernatant of hMOBs and hIOBs, respectively, that had been exposed to vibration. The cells that were exposed to vibration were subjected to 0 or 90 Hz vibration for 30 min and the supernatant was collected 24 h later. Significant differences between different vibrational frequency groups are indicated by * and ** (p < 0.05, Kruskal-Wallis post-test).
Fig. 4
Fig. 4
The diagram represents the effects of low-magnitude, high-frequency mechanical vibration on human mandible osteoblasts.
See this image and copyright information in PMC

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