Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Nov 27;18(2):533-541.
doi: 10.1007/s40200-019-00464-5. eCollection 2019 Dec.

Erythrocyte glucose-6-phosphate dehydrogenase activity and risk of gestational diabetes

Parvaneh Asadi  1 , Mahmood Vessal  1 , Marjan Khorsand  2 , Mohammad Ali Takhshid  2
Affiliations

Erythrocyte glucose-6-phosphate dehydrogenase activity and risk of gestational diabetes

Parvaneh Asadi et al. J Diabetes Metab Disord. .

Abstract

Purpose: Glucose-6-phosphate dehydrogenase (G6PD) is the regulating enzyme in the pentose phosphate pathway. A link between the activity of G6PD and diabetes mellitus has previously been reported. The association of G6PD activity with the pathogenesis of gestational diabetes mellitus (GDM) has not yet been investigated. The aim of the present study was to investigate the association of erythrocyte G6PD activity with major characteristics of GDM.

Methods: This case-control study was conducted at Hafez Hospital, Shiraz University of Medical Sciences, Shiraz, Iran from March to November 2017. Eighty-four age-matched pregnant women including GDM (n = 33), impaired glucose tolerance (IGT; n = 7), and normal glucose tolerance (NGT; n = 44) subjects were enrolled in this study. The levels of erythrocyte G6PD activity, fasting plasma glucose (FPG), insulin, malondialdehyde (MDA), and ferric reducing power (FRAP) of serum were measured. The level of homeostasis model for the assessment of insulin resistance (HOMA-IR) was calculated. The data were analyzed using SPSS software. P < 0.05 was considered statistically significant.

Results: The values of FPG, insulin, HOMA-IR, G6PD activity, and FRAP were significantly higher in GDM patients compared to NGT subjects. G6PD activity was correlated with FPG ((r = 0.224; P = 0.041). Binary logistic regression analysis revealed independent association of body mass index >25.88 [OR = 3.23, 95% CI 1.071-9.75, P = 0.037], HOMA- IR >2.33 [OR = 7.15, 95% CI 2.26-22.56, P < 0.001], and G6PD activity>21.17 U/g Hb [OR = 4.63, 95% CI 1.49-14.38, P = 0.008] with an increased risk of GDM. No significant change was observed among serum MDA levels in the three groups.

Conclusion: The findings demonstrate that increased G6PD activity is positively associated with the risk of GDM.

Keywords: Gestational diabetes; Glucose-6-phosphate dehydrogenase; Insulin resistance; Oxidative stress.

PubMed Disclaimer

Conflict of interest statement

Conflict of interestNone declared.

Figures

Fig. 1
Fig. 1
The graphs illustrate oral glucose tolerance test (OGTT) curves (a) and the area under the curve (AUC) of glucose levels OGTT (b) of normal glucose tolerance (NGT), impaired glucose tolerance (IGT) and gestational diabetes mellitus (GDM) subjects during the 100-g OGTT. Statistical differences were observed among NGT, IGT, and GDM groups (One-Way ANOVA followed by LSD post hoc test). Data are presented as means ± SD
Fig. 2
Fig. 2
Receiver operator characteristic curves were used to compare the performance of HOMA-IR, BMI, and G6PD activity in the diagnosis of GDM, BMI, body mass index; HOMA-IR, homeostasis model assessment index for insulin resistance; G6PD, Glucose-6-phosphate dehydrogenase; AUC, area under the curve
See this image and copyright information in PMC

References

    1. Coustan DR. Gestational diabetes mellitus. Clin Chem. 2013;59:1310–1321. doi: 10.1373/clinchem.2013.203331. - DOI - PubMed
    1. Farahvar S, Walfisch A, Sheiner E. Gestational diabetes risk factors and long-term consequences for both mother and offspring: a literature review. Expert Rev Endocrinol Metab. 2019;14:63–74. doi: 10.1080/17446651.2018.1476135. - DOI - PubMed
    1. Wang YP, Zhou LS, Zhao YZ, Wang SW, Chen LL, Liu LX, Ling ZQ, Hu FJ, Sun YP, Zhang JY, Yang C, Yang Y, Xiong Y, Guan KL, Ye D. Regulation of G6PD acetylation by SIRT2 and KAT9 modulates NADPH homeostasis and cell survival during oxidative stress. EMBO J. 2014;33:1304–1320. - PMC - PubMed
    1. Gómez-Manzo Saúl, Marcial-Quino Jaime, Vanoye-Carlo America, Serrano-Posada Hugo, Ortega-Cuellar Daniel, González-Valdez Abigail, Castillo-Rodríguez Rosa, Hernández-Ochoa Beatriz, Sierra-Palacios Edgar, Rodríguez-Bustamante Eduardo, Arreguin-Espinosa Roberto. Glucose-6-Phosphate Dehydrogenase: Update and Analysis of New Mutations around the World. International Journal of Molecular Sciences. 2016;17(12):2069. doi: 10.3390/ijms17122069. - DOI - PMC - PubMed
    1. Glucose-6-phosphate dehydrogenase deficiency. WHO Working Group. Bull World Health Organ. 1989;67:601–11. - PMC - PubMed

LinkOut - more resources